Decreased expression of glucose and peptide transporters in rat remnant kidney.

Nobuhiko Nakamura, Satohiro Masuda, Kazushige Takahashi, Hideyuki Saito, Masahiro Okuda, Ken ichi Inui

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The loss of renal mass induces tubular hypertrophy as well as glomerular sclerosis and results in the end stage of renal disease. However, there is little information about adaptation of tubular glucose and peptide reabsorption under conditions of chronic renal failure. In the present study, we performed functional and molecular analyses focused on the tubular reabsorption of filtered glucose and small peptides using 5/6 nephrectomized rats at 16 weeks, as a model of chronic renal failure. Sixteen weeks after 5/6 nephrectomy or sham treatment, the brush-border membranes and total RNA were obtained from the renal cortex to evaluate the uptake of Na(+) gradient-dependent D-glucose and H(+) gradient-dependent glycylsarcosine. The amounts of SGLT and PEPT mRNA levels were quantified by competitive PCR. The urinary glucose/creatinine ratio was markedly higher in nephrectomized rats than in sham-operated controls. Na(+)-dependent glucose uptake by the isolated renal brush-border membrane vesicles was markedly decreased in nephrectomized rats compared with that in sham-operated controls. However, H(+)-dependent peptide transport, another secondary active transport system in the brush-border membranes, was maintained. In addition, kinetic analysis revealed that both SGLT1 (high-affinity type)- and SGLT2 (low-affinity type)-mediated Na(+)/glucose uptake had markedly decreased Vmax values, but not Km values. Furthermore, competitive PCR demonstrated that the mRNA expression levels of SGLT2, PEPT1 and PEPT2, but not SGLT1, were markedly depressed. These findings suggested that loss of SGLT2 during chronic renal failure implies a high risk of renal glucosuria.

Original languageEnglish
Pages (from-to)41-47
Number of pages7
JournalDrug metabolism and pharmacokinetics
Volume19
Issue number1
DOIs
Publication statusPublished - Feb 2004

Fingerprint

Facilitative Glucose Transport Proteins
Kidney
Glucose
Chronic Kidney Failure
Microvilli
Peptides
Membranes
Renal Glycosuria
Polymerase Chain Reaction
Messenger RNA
Active Biological Transport
Sclerosis
Nephrectomy
Hypertrophy
peptide permease
Creatinine
Placebos
RNA

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science
  • Pharmacology (medical)

Cite this

Nakamura, N., Masuda, S., Takahashi, K., Saito, H., Okuda, M., & Inui, K. I. (2004). Decreased expression of glucose and peptide transporters in rat remnant kidney. Drug metabolism and pharmacokinetics, 19(1), 41-47. https://doi.org/10.2133/dmpk.19.41

Decreased expression of glucose and peptide transporters in rat remnant kidney. / Nakamura, Nobuhiko; Masuda, Satohiro; Takahashi, Kazushige; Saito, Hideyuki; Okuda, Masahiro; Inui, Ken ichi.

In: Drug metabolism and pharmacokinetics, Vol. 19, No. 1, 02.2004, p. 41-47.

Research output: Contribution to journalArticle

Nakamura, N, Masuda, S, Takahashi, K, Saito, H, Okuda, M & Inui, KI 2004, 'Decreased expression of glucose and peptide transporters in rat remnant kidney.', Drug metabolism and pharmacokinetics, vol. 19, no. 1, pp. 41-47. https://doi.org/10.2133/dmpk.19.41
Nakamura, Nobuhiko ; Masuda, Satohiro ; Takahashi, Kazushige ; Saito, Hideyuki ; Okuda, Masahiro ; Inui, Ken ichi. / Decreased expression of glucose and peptide transporters in rat remnant kidney. In: Drug metabolism and pharmacokinetics. 2004 ; Vol. 19, No. 1. pp. 41-47.
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