TY - JOUR
T1 - Decreased Lamin B1 Levels Affect Gene Positioning and Expression in Postmitotic Neurons
AU - Noguchi, Azumi
AU - Ito, Kenji
AU - Uosaki, Yuichi
AU - Ideta-Otsuka, Maky
AU - Igarashi, Katsuhide
AU - Nakashima, Hideyuki
AU - Kakizaki, Toshikazu
AU - Kaneda, Ruri
AU - Uosaki, Hideki
AU - Yanagawa, Yuchio
AU - Nakashima, Kinichi
AU - Arakawa, Hirokazu
AU - Takizawa, Takumi
N1 - Funding Information:
This work was supported by a JSPS Grant-in-Aid for Scientific Research on Innovative Areas [grant number 23114714 ]; and JST Strategic International Research Cooperative Program , SICP ( FY2012-2014 ).
Publisher Copyright:
© 2021 Elsevier B.V. and Japan Neuroscience Society
PY - 2021/12
Y1 - 2021/12
N2 - Gene expression programs and concomitant chromatin regulation change dramatically during the maturation of postmitotic neurons. Subnuclear positioning of gene loci is relevant to transcriptional regulation. However, little is known about subnuclear genome positioning in neuronal maturation. Using cultured murine hippocampal neurons, we found genomic locus 14qD2 to be enriched with genes that are upregulated during neuronal maturation. Reportedly, the locus is homologous to human 8p21.3, which has been extensively studied in neuropsychiatry and neurodegenerative diseases. Mapping of the 14qD2 locus in the nucleus revealed that it was relocated from the nuclear periphery to the interior. Moreover, we found a concomitant decrease in lamin B1 expression. Overexpression of lamin B1 in neurons using a lentiviral vector prevented the relocation of the 14qD2 locus and repressed the transcription of the Egr3 gene on this locus. Taken together, our results suggest that reduced lamin B1 expression during the maturation of neurons is important for appropriate subnuclear positioning of the genome and transcriptional programs.
AB - Gene expression programs and concomitant chromatin regulation change dramatically during the maturation of postmitotic neurons. Subnuclear positioning of gene loci is relevant to transcriptional regulation. However, little is known about subnuclear genome positioning in neuronal maturation. Using cultured murine hippocampal neurons, we found genomic locus 14qD2 to be enriched with genes that are upregulated during neuronal maturation. Reportedly, the locus is homologous to human 8p21.3, which has been extensively studied in neuropsychiatry and neurodegenerative diseases. Mapping of the 14qD2 locus in the nucleus revealed that it was relocated from the nuclear periphery to the interior. Moreover, we found a concomitant decrease in lamin B1 expression. Overexpression of lamin B1 in neurons using a lentiviral vector prevented the relocation of the 14qD2 locus and repressed the transcription of the Egr3 gene on this locus. Taken together, our results suggest that reduced lamin B1 expression during the maturation of neurons is important for appropriate subnuclear positioning of the genome and transcriptional programs.
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U2 - 10.1016/j.neures.2021.05.011
DO - 10.1016/j.neures.2021.05.011
M3 - Article
C2 - 34058264
AN - SCOPUS:85107328598
VL - 173
SP - 22
EP - 33
JO - Neuroscience Research
JF - Neuroscience Research
SN - 0168-0102
ER -
