Abstract
The relationship between in vivo biodistribution of 6-deoxy-6-[ 18F]fluoro-L-ascorbic acid (18F-DFA) and the content of tissue glutathione (GSH) was investigated in Wistar male rats. Following intravenous administration of 18F-DFA, the accumulation of radioactivity in most tissues, including the adrenal glands, liver and brain, was significantly reduced together with a decrease in the content of GSH by preloading of diethyl maleate (DEM) which depletes cellular GSH. Similar decreased uptake was also observed in the distribution of L-[1- 14C]ascorbic acid (14C-AA) after DEM treatment. The possible biological mechanisms, including competition with endogenous AA and ascorbate recycling, that modulate the uptake and accumulation into tissues of 18F-DFA and 14C-AA in GSH-deficient rats are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 1943-1947 |
| Number of pages | 5 |
| Journal | Biological and Pharmaceutical Bulletin |
| Volume | 28 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 1 2005 |
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All Science Journal Classification (ASJC) codes
- Pharmacology
- Pharmaceutical Science
Cite this
Decreased tissue accumulation of 6-deoxy-6-[18F]fluoro-L- ascorbic acid in glutathione-deficient rats induced by administration of diethyl maleate. / Yamamoto, Fumihiko; Kaneshiro, Tetsuo; Kato, Hiroharu; Mukai, Takahiro; Kuwabara, Yasuo; Honda, Hiroshi; Maeda, Minoru.
In: Biological and Pharmaceutical Bulletin, Vol. 28, No. 10, 01.10.2005, p. 1943-1947.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Decreased tissue accumulation of 6-deoxy-6-[18F]fluoro-L- ascorbic acid in glutathione-deficient rats induced by administration of diethyl maleate
AU - Yamamoto, Fumihiko
AU - Kaneshiro, Tetsuo
AU - Kato, Hiroharu
AU - Mukai, Takahiro
AU - Kuwabara, Yasuo
AU - Honda, Hiroshi
AU - Maeda, Minoru
PY - 2005/10/1
Y1 - 2005/10/1
N2 - The relationship between in vivo biodistribution of 6-deoxy-6-[ 18F]fluoro-L-ascorbic acid (18F-DFA) and the content of tissue glutathione (GSH) was investigated in Wistar male rats. Following intravenous administration of 18F-DFA, the accumulation of radioactivity in most tissues, including the adrenal glands, liver and brain, was significantly reduced together with a decrease in the content of GSH by preloading of diethyl maleate (DEM) which depletes cellular GSH. Similar decreased uptake was also observed in the distribution of L-[1- 14C]ascorbic acid (14C-AA) after DEM treatment. The possible biological mechanisms, including competition with endogenous AA and ascorbate recycling, that modulate the uptake and accumulation into tissues of 18F-DFA and 14C-AA in GSH-deficient rats are discussed.
AB - The relationship between in vivo biodistribution of 6-deoxy-6-[ 18F]fluoro-L-ascorbic acid (18F-DFA) and the content of tissue glutathione (GSH) was investigated in Wistar male rats. Following intravenous administration of 18F-DFA, the accumulation of radioactivity in most tissues, including the adrenal glands, liver and brain, was significantly reduced together with a decrease in the content of GSH by preloading of diethyl maleate (DEM) which depletes cellular GSH. Similar decreased uptake was also observed in the distribution of L-[1- 14C]ascorbic acid (14C-AA) after DEM treatment. The possible biological mechanisms, including competition with endogenous AA and ascorbate recycling, that modulate the uptake and accumulation into tissues of 18F-DFA and 14C-AA in GSH-deficient rats are discussed.
UR - http://www.scopus.com/inward/record.url?scp=26444451026&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=26444451026&partnerID=8YFLogxK
U2 - 10.1248/bpb.28.1943
DO - 10.1248/bpb.28.1943
M3 - Article
C2 - 16204951
AN - SCOPUS:26444451026
VL - 28
SP - 1943
EP - 1947
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
SN - 0918-6158
IS - 10
ER -