Decreased tissue accumulation of 6-deoxy-6-[18F]fluoro-L- ascorbic acid in glutathione-deficient rats induced by administration of diethyl maleate

Fumihiko Yamamoto; Tetsuo Kaneshiro; Hiroharu Kato; Takahiro Mukai; Yasuo Kuwabara; Hiroshi Honda; Minoru Maeda

doi:10.1248/bpb.28.1943

Decreased tissue accumulation of 6-deoxy-6-[¹⁸F]fluoro-L- ascorbic acid in glutathione-deficient rats induced by administration of diethyl maleate

Fumihiko Yamamoto, Tetsuo Kaneshiro, Hiroharu Kato, Takahiro Mukai, Yasuo Kuwabara, Hiroshi Honda, Minoru Maeda

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The relationship between in vivo biodistribution of 6-deoxy-6-[ ¹⁸F]fluoro-L-ascorbic acid (¹⁸F-DFA) and the content of tissue glutathione (GSH) was investigated in Wistar male rats. Following intravenous administration of ¹⁸F-DFA, the accumulation of radioactivity in most tissues, including the adrenal glands, liver and brain, was significantly reduced together with a decrease in the content of GSH by preloading of diethyl maleate (DEM) which depletes cellular GSH. Similar decreased uptake was also observed in the distribution of L-[1- ¹⁴C]ascorbic acid (¹⁴C-AA) after DEM treatment. The possible biological mechanisms, including competition with endogenous AA and ascorbate recycling, that modulate the uptake and accumulation into tissues of ¹⁸F-DFA and ¹⁴C-AA in GSH-deficient rats are discussed.

Original language	English
Pages (from-to)	1943-1947
Number of pages	5
Journal	Biological and Pharmaceutical Bulletin
Volume	28
Issue number	10
DOIs	https://doi.org/10.1248/bpb.28.1943
Publication status	Published - Oct 2005

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmaceutical Science

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title = "Decreased tissue accumulation of 6-deoxy-6-[18F]fluoro-L- ascorbic acid in glutathione-deficient rats induced by administration of diethyl maleate",

abstract = "The relationship between in vivo biodistribution of 6-deoxy-6-[ 18F]fluoro-L-ascorbic acid (18F-DFA) and the content of tissue glutathione (GSH) was investigated in Wistar male rats. Following intravenous administration of 18F-DFA, the accumulation of radioactivity in most tissues, including the adrenal glands, liver and brain, was significantly reduced together with a decrease in the content of GSH by preloading of diethyl maleate (DEM) which depletes cellular GSH. Similar decreased uptake was also observed in the distribution of L-[1- 14C]ascorbic acid (14C-AA) after DEM treatment. The possible biological mechanisms, including competition with endogenous AA and ascorbate recycling, that modulate the uptake and accumulation into tissues of 18F-DFA and 14C-AA in GSH-deficient rats are discussed.",

author = "Fumihiko Yamamoto and Tetsuo Kaneshiro and Hiroharu Kato and Takahiro Mukai and Yasuo Kuwabara and Hiroshi Honda and Minoru Maeda",

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AU - Yamamoto, Fumihiko

AU - Kaneshiro, Tetsuo

AU - Kato, Hiroharu

AU - Mukai, Takahiro

AU - Kuwabara, Yasuo

AU - Honda, Hiroshi

AU - Maeda, Minoru

PY - 2005/10

Y1 - 2005/10

N2 - The relationship between in vivo biodistribution of 6-deoxy-6-[ 18F]fluoro-L-ascorbic acid (18F-DFA) and the content of tissue glutathione (GSH) was investigated in Wistar male rats. Following intravenous administration of 18F-DFA, the accumulation of radioactivity in most tissues, including the adrenal glands, liver and brain, was significantly reduced together with a decrease in the content of GSH by preloading of diethyl maleate (DEM) which depletes cellular GSH. Similar decreased uptake was also observed in the distribution of L-[1- 14C]ascorbic acid (14C-AA) after DEM treatment. The possible biological mechanisms, including competition with endogenous AA and ascorbate recycling, that modulate the uptake and accumulation into tissues of 18F-DFA and 14C-AA in GSH-deficient rats are discussed.

AB - The relationship between in vivo biodistribution of 6-deoxy-6-[ 18F]fluoro-L-ascorbic acid (18F-DFA) and the content of tissue glutathione (GSH) was investigated in Wistar male rats. Following intravenous administration of 18F-DFA, the accumulation of radioactivity in most tissues, including the adrenal glands, liver and brain, was significantly reduced together with a decrease in the content of GSH by preloading of diethyl maleate (DEM) which depletes cellular GSH. Similar decreased uptake was also observed in the distribution of L-[1- 14C]ascorbic acid (14C-AA) after DEM treatment. The possible biological mechanisms, including competition with endogenous AA and ascorbate recycling, that modulate the uptake and accumulation into tissues of 18F-DFA and 14C-AA in GSH-deficient rats are discussed.

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Decreased tissue accumulation of 6-deoxy-6-[¹⁸F]fluoro-L- ascorbic acid in glutathione-deficient rats induced by administration of diethyl maleate

Abstract

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