Decreased triglyceride-rich lipoproteins in transgenic skinny mice overexpressing leptin

Naoki Matsuoka, Yoshihiro Ogawa, Hiroaki Masuzaki, Ken Ebihara, Megumi Aizawa-Abe, Noriko Satoh, Eiichiro Ishikawa, Yukio Fujisawa, Atsushi Kosaki, Kazunori Yamada, Hideshi Kuzuya, Kazuwa Nakao

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Leptin is an adipocyte-derived circulating satiety factor with a variety of biological effects. Evidence has accumulated suggesting that leptin may modulate glucose and lipid metabolism. In the present study, we examined lipid metabolism in transgenic skinny mice with elevated plasma leptin concentrations. The plasma concentrations of triglycerides and free fatty acids in transgenic skinny mice were 71.5 (P < 0.01) and 89.1% (P < 0.05) of those in their nontransgenic littermates, respectively. Separation of plasma into lipoprotein classes by ultracentrifugation revealed that very low density lipoprotein-triglyceride concentrations were markedly reduced in transgenic skinny mice relative to the controls. The clearance of triglycerides estimated by a fat-loading test was enhanced in transgenic skinny mice; the triglyceride concentration in transgenic skinny mice 3 h after fat loading was 39.7% (P < 0.05) of that of their nontransgenic littermates. Postheparin plasma lipoprotein lipase activity increased 1.4-fold (P < 0.05) in transgenic skinny mice. Our data demonstrated a significant reduction in plasma triglyceride concentrations, accompanied by increased lipoprotein lipase activity in transgenic skinny mice overexpressing leptin, suggesting that leptin plays a role in long-term triglyceride metabolism.

Original languageEnglish
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume280
Issue number2 43-2
Publication statusPublished - Feb 1 2001

Fingerprint

Leptin
Transgenic Mice
Lipoproteins
Triglycerides
Lipoprotein Lipase
Lipid Metabolism
Fats
Ultracentrifugation
Nonesterified Fatty Acids
Adipocytes
Glucose

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Matsuoka, N., Ogawa, Y., Masuzaki, H., Ebihara, K., Aizawa-Abe, M., Satoh, N., ... Nakao, K. (2001). Decreased triglyceride-rich lipoproteins in transgenic skinny mice overexpressing leptin. American Journal of Physiology - Endocrinology and Metabolism, 280(2 43-2).

Decreased triglyceride-rich lipoproteins in transgenic skinny mice overexpressing leptin. / Matsuoka, Naoki; Ogawa, Yoshihiro; Masuzaki, Hiroaki; Ebihara, Ken; Aizawa-Abe, Megumi; Satoh, Noriko; Ishikawa, Eiichiro; Fujisawa, Yukio; Kosaki, Atsushi; Yamada, Kazunori; Kuzuya, Hideshi; Nakao, Kazuwa.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 280, No. 2 43-2, 01.02.2001.

Research output: Contribution to journalArticle

Matsuoka, N, Ogawa, Y, Masuzaki, H, Ebihara, K, Aizawa-Abe, M, Satoh, N, Ishikawa, E, Fujisawa, Y, Kosaki, A, Yamada, K, Kuzuya, H & Nakao, K 2001, 'Decreased triglyceride-rich lipoproteins in transgenic skinny mice overexpressing leptin', American Journal of Physiology - Endocrinology and Metabolism, vol. 280, no. 2 43-2.
Matsuoka, Naoki ; Ogawa, Yoshihiro ; Masuzaki, Hiroaki ; Ebihara, Ken ; Aizawa-Abe, Megumi ; Satoh, Noriko ; Ishikawa, Eiichiro ; Fujisawa, Yukio ; Kosaki, Atsushi ; Yamada, Kazunori ; Kuzuya, Hideshi ; Nakao, Kazuwa. / Decreased triglyceride-rich lipoproteins in transgenic skinny mice overexpressing leptin. In: American Journal of Physiology - Endocrinology and Metabolism. 2001 ; Vol. 280, No. 2 43-2.
@article{622e892424cf40519e2303b57c3560f6,
title = "Decreased triglyceride-rich lipoproteins in transgenic skinny mice overexpressing leptin",
abstract = "Leptin is an adipocyte-derived circulating satiety factor with a variety of biological effects. Evidence has accumulated suggesting that leptin may modulate glucose and lipid metabolism. In the present study, we examined lipid metabolism in transgenic skinny mice with elevated plasma leptin concentrations. The plasma concentrations of triglycerides and free fatty acids in transgenic skinny mice were 71.5 (P < 0.01) and 89.1{\%} (P < 0.05) of those in their nontransgenic littermates, respectively. Separation of plasma into lipoprotein classes by ultracentrifugation revealed that very low density lipoprotein-triglyceride concentrations were markedly reduced in transgenic skinny mice relative to the controls. The clearance of triglycerides estimated by a fat-loading test was enhanced in transgenic skinny mice; the triglyceride concentration in transgenic skinny mice 3 h after fat loading was 39.7{\%} (P < 0.05) of that of their nontransgenic littermates. Postheparin plasma lipoprotein lipase activity increased 1.4-fold (P < 0.05) in transgenic skinny mice. Our data demonstrated a significant reduction in plasma triglyceride concentrations, accompanied by increased lipoprotein lipase activity in transgenic skinny mice overexpressing leptin, suggesting that leptin plays a role in long-term triglyceride metabolism.",
author = "Naoki Matsuoka and Yoshihiro Ogawa and Hiroaki Masuzaki and Ken Ebihara and Megumi Aizawa-Abe and Noriko Satoh and Eiichiro Ishikawa and Yukio Fujisawa and Atsushi Kosaki and Kazunori Yamada and Hideshi Kuzuya and Kazuwa Nakao",
year = "2001",
month = "2",
day = "1",
language = "English",
volume = "280",
journal = "American Journal of Physiology - Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "2 43-2",

}

TY - JOUR

T1 - Decreased triglyceride-rich lipoproteins in transgenic skinny mice overexpressing leptin

AU - Matsuoka, Naoki

AU - Ogawa, Yoshihiro

AU - Masuzaki, Hiroaki

AU - Ebihara, Ken

AU - Aizawa-Abe, Megumi

AU - Satoh, Noriko

AU - Ishikawa, Eiichiro

AU - Fujisawa, Yukio

AU - Kosaki, Atsushi

AU - Yamada, Kazunori

AU - Kuzuya, Hideshi

AU - Nakao, Kazuwa

PY - 2001/2/1

Y1 - 2001/2/1

N2 - Leptin is an adipocyte-derived circulating satiety factor with a variety of biological effects. Evidence has accumulated suggesting that leptin may modulate glucose and lipid metabolism. In the present study, we examined lipid metabolism in transgenic skinny mice with elevated plasma leptin concentrations. The plasma concentrations of triglycerides and free fatty acids in transgenic skinny mice were 71.5 (P < 0.01) and 89.1% (P < 0.05) of those in their nontransgenic littermates, respectively. Separation of plasma into lipoprotein classes by ultracentrifugation revealed that very low density lipoprotein-triglyceride concentrations were markedly reduced in transgenic skinny mice relative to the controls. The clearance of triglycerides estimated by a fat-loading test was enhanced in transgenic skinny mice; the triglyceride concentration in transgenic skinny mice 3 h after fat loading was 39.7% (P < 0.05) of that of their nontransgenic littermates. Postheparin plasma lipoprotein lipase activity increased 1.4-fold (P < 0.05) in transgenic skinny mice. Our data demonstrated a significant reduction in plasma triglyceride concentrations, accompanied by increased lipoprotein lipase activity in transgenic skinny mice overexpressing leptin, suggesting that leptin plays a role in long-term triglyceride metabolism.

AB - Leptin is an adipocyte-derived circulating satiety factor with a variety of biological effects. Evidence has accumulated suggesting that leptin may modulate glucose and lipid metabolism. In the present study, we examined lipid metabolism in transgenic skinny mice with elevated plasma leptin concentrations. The plasma concentrations of triglycerides and free fatty acids in transgenic skinny mice were 71.5 (P < 0.01) and 89.1% (P < 0.05) of those in their nontransgenic littermates, respectively. Separation of plasma into lipoprotein classes by ultracentrifugation revealed that very low density lipoprotein-triglyceride concentrations were markedly reduced in transgenic skinny mice relative to the controls. The clearance of triglycerides estimated by a fat-loading test was enhanced in transgenic skinny mice; the triglyceride concentration in transgenic skinny mice 3 h after fat loading was 39.7% (P < 0.05) of that of their nontransgenic littermates. Postheparin plasma lipoprotein lipase activity increased 1.4-fold (P < 0.05) in transgenic skinny mice. Our data demonstrated a significant reduction in plasma triglyceride concentrations, accompanied by increased lipoprotein lipase activity in transgenic skinny mice overexpressing leptin, suggesting that leptin plays a role in long-term triglyceride metabolism.

UR - http://www.scopus.com/inward/record.url?scp=0035024344&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035024344&partnerID=8YFLogxK

M3 - Article

VL - 280

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

SN - 0363-6135

IS - 2 43-2

ER -