Deficiency of starch synthase IIIa and IVb alters starch granule morphology from polyhedral to spherical in rice endosperm

Yoshiko Toyosawa, Yasushi Kawagoe, Ryo Matsushima, Naoko Crofts, Masahiro Ogawa, Masako Fukuda, Toshihiro Kumamaru, Yozo Okazaki, Miyako Kusano, Kazuki Saito, Kiminori Toyooka, Mayuko Sato, Yongfeng Ai, Jay Lin Jane, Yasunori Nakamura, Naoko Fujita

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Starch granule morphology differs markedly among plant species. However, the mechanisms controlling starch granule morphology have not been elucidated. Rice (Oryza sativa) endosperm produces characteristic compound-type granules containing dozens of polyhedral starch granules within an amyloplast. Some other cereal species produce simple-type granules, in which only one starch granule is present per amyloplast. A double mutant rice deficient in the starch synthase (SS) genes SSIIIa and SSIVb (ss3a ss4b) produced spherical starch granules, whereas the parental single mutants produced polyhedral starch granules similar to the wild type. The ss3a ss4b amyloplasts contained compound-type starch granules during early developmental stages, and spherical granules were separated from each other during subsequent amyloplast development and seed dehydration. Analysis of glucan chain length distribution identified overlapping roles for SSIIIa and SSIVb in amylopectin chain synthesis, with a degree of polymerization of 42 or greater. Confocal fluorescence microscopy and immunoelectron microscopy of wild-type developing rice seeds revealed that the majority of SSIVb was localized between starch granules. Therefore, we propose that SSIIIa and SSIVb have crucial roles in determining starch granule morphology and in maintaining the amyloplast envelope structure. We present a model of spherical starch granule production.

Original languageEnglish
Pages (from-to)1255-1270
Number of pages16
JournalPlant physiology
Volume170
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

Fingerprint

Starch Synthase
starch synthase
Endosperm
starch granules
Starch
endosperm
rice
amyloplasts
Plastids
granules
Seeds
Oryza
Amylopectin
mutants
Glucans
Immunoelectron Microscopy
amylopectin
glucans
fluorescence microscopy
seeds

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Toyosawa, Y., Kawagoe, Y., Matsushima, R., Crofts, N., Ogawa, M., Fukuda, M., ... Fujita, N. (2016). Deficiency of starch synthase IIIa and IVb alters starch granule morphology from polyhedral to spherical in rice endosperm. Plant physiology, 170(3), 1255-1270. https://doi.org/10.1104/pp.15.01232

Deficiency of starch synthase IIIa and IVb alters starch granule morphology from polyhedral to spherical in rice endosperm. / Toyosawa, Yoshiko; Kawagoe, Yasushi; Matsushima, Ryo; Crofts, Naoko; Ogawa, Masahiro; Fukuda, Masako; Kumamaru, Toshihiro; Okazaki, Yozo; Kusano, Miyako; Saito, Kazuki; Toyooka, Kiminori; Sato, Mayuko; Ai, Yongfeng; Jane, Jay Lin; Nakamura, Yasunori; Fujita, Naoko.

In: Plant physiology, Vol. 170, No. 3, 01.03.2016, p. 1255-1270.

Research output: Contribution to journalArticle

Toyosawa, Y, Kawagoe, Y, Matsushima, R, Crofts, N, Ogawa, M, Fukuda, M, Kumamaru, T, Okazaki, Y, Kusano, M, Saito, K, Toyooka, K, Sato, M, Ai, Y, Jane, JL, Nakamura, Y & Fujita, N 2016, 'Deficiency of starch synthase IIIa and IVb alters starch granule morphology from polyhedral to spherical in rice endosperm', Plant physiology, vol. 170, no. 3, pp. 1255-1270. https://doi.org/10.1104/pp.15.01232
Toyosawa, Yoshiko ; Kawagoe, Yasushi ; Matsushima, Ryo ; Crofts, Naoko ; Ogawa, Masahiro ; Fukuda, Masako ; Kumamaru, Toshihiro ; Okazaki, Yozo ; Kusano, Miyako ; Saito, Kazuki ; Toyooka, Kiminori ; Sato, Mayuko ; Ai, Yongfeng ; Jane, Jay Lin ; Nakamura, Yasunori ; Fujita, Naoko. / Deficiency of starch synthase IIIa and IVb alters starch granule morphology from polyhedral to spherical in rice endosperm. In: Plant physiology. 2016 ; Vol. 170, No. 3. pp. 1255-1270.
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