Defining raft domains in the plasma membrane

Akihiro Kusumi, Takahiro K. Fujiwara, Taka A. Tsunoyama, Rinshi S. Kasai, An An Liu, Koichiro M. Hirosawa, Masanao Kinoshita, Nobuaki Matsumori, Naoko Komura, Hiromune Ando, Kenichi G.N. Suzuki

Research output: Contribution to journalReview article

Abstract

Many plasma membrane (PM) functions depend on the cholesterol concentration in the PM in strikingly nonlinear, cooperative ways: fully functional in the presence of physiological cholesterol levels (35~45 mol%), and nonfunctional below 25 mol% cholesterol; namely, still in the presence of high concentrations of cholesterol. This suggests the involvement of cholesterol-based complexes/domains formed cooperatively. In this review, by examining the results obtained by using fluorescent lipid analogs and avoiding the trap of circular logic, often found in the raft literature, we point out the fundamental similarities of liquid-ordered (Lo)-phase domains in giant unilamellar vesicles, Lo-phase-like domains formed at lower temperatures in giant PM vesicles, and detergent-resistant membranes: these domains are formed by cooperative interactions of cholesterol, saturated acyl chains, and unsaturated acyl chains, in the presence of >25 mol% cholesterol. The literature contains evidence, indicating that the domains formed by the same basic cooperative molecular interactions exist and play essential roles in signal transduction in the PM. Therefore, as a working definition, we propose that raft domains in the PM are liquid-like molecular complexes/domains formed by cooperative interactions of cholesterol with saturated acyl chains as well as unsaturated acyl chains, due to saturated acyl chains' weak multiple accommodating interactions with cholesterol and cholesterol's low miscibility with unsaturated acyl chains and TM proteins. Molecules move within raft domains and exchange with those in the bulk PM. We provide a logically established collection of fluorescent lipid probes that preferentially partition into raft and non-raft domains, as defined here, in the PM.

Original languageEnglish
Pages (from-to)106-137
Number of pages32
JournalTraffic
Volume21
Issue number1
DOIs
Publication statusPublished - Jan 1 2020

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Cell membranes
Cholesterol
Cell Membrane
Liquids
Lipids
Unilamellar Liposomes
Signal transduction
Molecular interactions
Fluorescent Dyes
Detergents
Signal Transduction
Solubility
Membranes
Molecules
Temperature

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Kusumi, A., Fujiwara, T. K., Tsunoyama, T. A., Kasai, R. S., Liu, A. A., Hirosawa, K. M., ... Suzuki, K. G. N. (2020). Defining raft domains in the plasma membrane. Traffic, 21(1), 106-137. https://doi.org/10.1111/tra.12718

Defining raft domains in the plasma membrane. / Kusumi, Akihiro; Fujiwara, Takahiro K.; Tsunoyama, Taka A.; Kasai, Rinshi S.; Liu, An An; Hirosawa, Koichiro M.; Kinoshita, Masanao; Matsumori, Nobuaki; Komura, Naoko; Ando, Hiromune; Suzuki, Kenichi G.N.

In: Traffic, Vol. 21, No. 1, 01.01.2020, p. 106-137.

Research output: Contribution to journalReview article

Kusumi, A, Fujiwara, TK, Tsunoyama, TA, Kasai, RS, Liu, AA, Hirosawa, KM, Kinoshita, M, Matsumori, N, Komura, N, Ando, H & Suzuki, KGN 2020, 'Defining raft domains in the plasma membrane', Traffic, vol. 21, no. 1, pp. 106-137. https://doi.org/10.1111/tra.12718
Kusumi A, Fujiwara TK, Tsunoyama TA, Kasai RS, Liu AA, Hirosawa KM et al. Defining raft domains in the plasma membrane. Traffic. 2020 Jan 1;21(1):106-137. https://doi.org/10.1111/tra.12718
Kusumi, Akihiro ; Fujiwara, Takahiro K. ; Tsunoyama, Taka A. ; Kasai, Rinshi S. ; Liu, An An ; Hirosawa, Koichiro M. ; Kinoshita, Masanao ; Matsumori, Nobuaki ; Komura, Naoko ; Ando, Hiromune ; Suzuki, Kenichi G.N. / Defining raft domains in the plasma membrane. In: Traffic. 2020 ; Vol. 21, No. 1. pp. 106-137.
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