Defining the dynamin-based ring organizing center on the peroxisome-dividing machinery isolated from Cyanidioschyzon merolae

Yuuta Imoto, Yuichi Abe, Kanji Okumoto, Masanori Honsho, Haruko Kuroiwa, Tsuneyoshi Kuroiwa, Yukio Fujiki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Organelle division is executed through contraction of a ring-shaped supramolecular dividing machinery. A core component of the machinery is the dynamin-based ring conserved during the division of mitochondrion, plastid and peroxisome. Here, using isolated peroxisome-dividing (POD) machinery from a unicellular red algae, Cyanidioschyzon merolae, we identified a dynamin-based ring organizing center (DOC) that acts as an initiation point for formation of the dynamin-based ring. C. merolae contains a single peroxisome, the division of which can be highly synchronized by light-dark stimulation; thus, intact POD machinery can be isolated in bulk. Dynamin-based ring homeostasis is maintained by the turnover of the GTP-bound form of the dynamin-related protein Dnm1 between the cytosol and division machinery via the DOC. A single DOC is formed on the POD machinery with a diameter of 500-700 nm, and the dynamin-based ring is unidirectionally elongated from the DOC in a manner that is dependent on GTP concentration. During the later step of membrane fission, the second DOC is formed and constructs the double dynamin-based ring to make the machinery thicker. These findings provide new insights to define fundamental mechanisms underlying the dynamin-based membrane fission in eukaryotic cells.

Original languageEnglish
Pages (from-to)853-867
Number of pages15
JournalJournal of cell science
Volume130
Issue number5
DOIs
Publication statusPublished - Jan 1 2017

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Dynamins
Peroxisomes
Guanosine Triphosphate
Rhodophyta
Plastids
Membranes
Eukaryotic Cells
Organelles
Cytosol
Mitochondria

All Science Journal Classification (ASJC) codes

  • Cell Biology

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Defining the dynamin-based ring organizing center on the peroxisome-dividing machinery isolated from Cyanidioschyzon merolae. / Imoto, Yuuta; Abe, Yuichi; Okumoto, Kanji; Honsho, Masanori; Kuroiwa, Haruko; Kuroiwa, Tsuneyoshi; Fujiki, Yukio.

In: Journal of cell science, Vol. 130, No. 5, 01.01.2017, p. 853-867.

Research output: Contribution to journalArticle

Imoto, Yuuta ; Abe, Yuichi ; Okumoto, Kanji ; Honsho, Masanori ; Kuroiwa, Haruko ; Kuroiwa, Tsuneyoshi ; Fujiki, Yukio. / Defining the dynamin-based ring organizing center on the peroxisome-dividing machinery isolated from Cyanidioschyzon merolae. In: Journal of cell science. 2017 ; Vol. 130, No. 5. pp. 853-867.
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