TY - JOUR
T1 - Defining the genome features of Escherichia albertii, an emerging enteropathogen closely related to Escherichia coli
AU - Ooka, Tadasuke
AU - Ogura, Yoshitoshi
AU - Katsura, Keisuke
AU - Seto, Kazuko
AU - Kobayashi, Hideki
AU - Kawano, Kimiko
AU - Tokuoka, Eisuke
AU - Furukawa, Masato
AU - Harada, Seiya
AU - Yoshino, Shuji
AU - Seto, Junji
AU - Ikeda, Tetsuya
AU - Yamaguchi, Keiji
AU - Murase, Kazunori
AU - Gotoh, Yasuhiro
AU - Imuta, Naoko
AU - Nishi, Junichiro
AU - Gomes, Tânia A.
AU - Beutin, Lothar
AU - Hayashi, Tetsuya
N1 - Funding Information:
Most of this work was conducted at the University of Miyazaki. The authors thank A. Yoshida, N. Kawano, N. Sakamoto, S. Yamamoto, Y. Inoue, H. Iguchi, and M. Shinbara for providing technical assistance. This work was supported by JSPS KAKENHI Grant Numbers [23790480, 25460539 to T.O., and 20310116 to T.H.], a Grant-in-Aid for Scientific Research in Innovative Areas “Genome Science” from the Ministry of Education, Culture, Sports, Science and Technology of Japan [221S0002 to T.H.], a grant from Kurozumi Medical Foundation to T.O., and the Integrated Research Project for Human and Veterinary Medicine of the University of Miyazaki.
Publisher Copyright:
©The Author(s) 2015. Published by Oxford University Press on behalf of the Society for.
PY - 2015
Y1 - 2015
N2 - Escherichia albertii is a recently recognized close relative of Escherichia coli. This emerging enteropathogen possesses a type III secretion system (T3SS) encoded by the locus of enterocyte effacement, similar to enteropathogenic and enterohemorrhagic E. coli (EPEC and EHEC). Shiga toxin-producing strains have also been identified. The genomic features of E. albertii, particularly differences from other Escherichia species, have not yet been well clarified.Here,wesequenced the genomeof 29 E. albertii strains (3 complete and 26 draft sequences) isolated frommultiple sources and performed intraspecies and intragenus genomic comparisons. The sizes of the E. albertii genomes range from 4.5 to 5.1Mb, smaller than those of E. coli strains. Intraspecies genomic comparisons identified five phylogroups of E. albertii. Intragenus genomic comparison revealed that the possible core genome of E. albertii comprises 3,250 genes, whereas that of the genus Escherichia comprises 1,345 genes. Our analysis further revealed several unique or notable genetic features of E. albertii, including those responsible for known biochemical features and virulence factors anda possibly active secondT3SSknownas ETT2(E. coliT3SS2) that is inactivated inE. coli.Althoughthisorganismhasbeenobserved to be nonmotile in vitro, genes for flagellar biosynthesis are fully conserved; chemotaxis-related genes have been selectively deleted. Based onthese results,wehave developed a nested polymerase chain reaction system to directly detect E. albertii.Our data define the genomic features of E. albertii and provide a valuable basis for future studies of this important emerging enteropathogen.
AB - Escherichia albertii is a recently recognized close relative of Escherichia coli. This emerging enteropathogen possesses a type III secretion system (T3SS) encoded by the locus of enterocyte effacement, similar to enteropathogenic and enterohemorrhagic E. coli (EPEC and EHEC). Shiga toxin-producing strains have also been identified. The genomic features of E. albertii, particularly differences from other Escherichia species, have not yet been well clarified.Here,wesequenced the genomeof 29 E. albertii strains (3 complete and 26 draft sequences) isolated frommultiple sources and performed intraspecies and intragenus genomic comparisons. The sizes of the E. albertii genomes range from 4.5 to 5.1Mb, smaller than those of E. coli strains. Intraspecies genomic comparisons identified five phylogroups of E. albertii. Intragenus genomic comparison revealed that the possible core genome of E. albertii comprises 3,250 genes, whereas that of the genus Escherichia comprises 1,345 genes. Our analysis further revealed several unique or notable genetic features of E. albertii, including those responsible for known biochemical features and virulence factors anda possibly active secondT3SSknownas ETT2(E. coliT3SS2) that is inactivated inE. coli.Althoughthisorganismhasbeenobserved to be nonmotile in vitro, genes for flagellar biosynthesis are fully conserved; chemotaxis-related genes have been selectively deleted. Based onthese results,wehave developed a nested polymerase chain reaction system to directly detect E. albertii.Our data define the genomic features of E. albertii and provide a valuable basis for future studies of this important emerging enteropathogen.
UR - http://www.scopus.com/inward/record.url?scp=84983780972&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84983780972&partnerID=8YFLogxK
U2 - 10.1093/gbe/evv211
DO - 10.1093/gbe/evv211
M3 - Article
C2 - 26537224
AN - SCOPUS:84983780972
SN - 1759-6653
VL - 7
SP - 3170
EP - 3179
JO - Genome Biology and Evolution
JF - Genome Biology and Evolution
IS - 12
ER -