Defining the genome features of Escherichia albertii, an emerging enteropathogen closely related to Escherichia coli

Tadasuke Ooka, Yoshitoshi Ogura, Keisuke Katsura, Kazuko Seto, Hideki Kobayashi, Kimiko Kawano, Eisuke Tokuoka, Masato Furukawa, Seiya Harada, Shuji Yoshino, Junji Seto, Tetsuya Ikeda, Keiji Yamaguchi, Kazunori Murase, Yasuhiro Gotoh, Naoko Imuta, Junichiro Nishi, Tânia A. Gomes, Lothar Beutin, Tetsuya Hayashi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Escherichia albertii is a recently recognized close relative of Escherichia coli. This emerging enteropathogen possesses a type III secretion system (T3SS) encoded by the locus of enterocyte effacement, similar to enteropathogenic and enterohemorrhagic E. coli (EPEC and EHEC). Shiga toxin-producing strains have also been identified. The genomic features of E. albertii, particularly differences from other Escherichia species, have not yet been well clarified.Here,wesequenced the genomeof 29 E. albertii strains (3 complete and 26 draft sequences) isolated frommultiple sources and performed intraspecies and intragenus genomic comparisons. The sizes of the E. albertii genomes range from 4.5 to 5.1Mb, smaller than those of E. coli strains. Intraspecies genomic comparisons identified five phylogroups of E. albertii. Intragenus genomic comparison revealed that the possible core genome of E. albertii comprises 3,250 genes, whereas that of the genus Escherichia comprises 1,345 genes. Our analysis further revealed several unique or notable genetic features of E. albertii, including those responsible for known biochemical features and virulence factors anda possibly active secondT3SSknownas ETT2(E. coliT3SS2) that is inactivated inE. coli.Althoughthisorganismhasbeenobserved to be nonmotile in vitro, genes for flagellar biosynthesis are fully conserved; chemotaxis-related genes have been selectively deleted. Based onthese results,wehave developed a nested polymerase chain reaction system to directly detect E. albertii.Our data define the genomic features of E. albertii and provide a valuable basis for future studies of this important emerging enteropathogen.

Original languageEnglish
Pages (from-to)3170-3179
Number of pages10
JournalGenome Biology and Evolution
Volume7
Issue number12
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Escherichia albertii
enteropathogens
Escherichia
genomics
genome
Genome
Escherichia coli
gene
Genes
Shiga Toxin
Enterohemorrhagic Escherichia coli
Enteropathogenic Escherichia coli
type III secretion system
Enterocytes
Virulence Factors
Chemotaxis
chemotaxis
virulence
secretion
polymerase chain reaction

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Defining the genome features of Escherichia albertii, an emerging enteropathogen closely related to Escherichia coli. / Ooka, Tadasuke; Ogura, Yoshitoshi; Katsura, Keisuke; Seto, Kazuko; Kobayashi, Hideki; Kawano, Kimiko; Tokuoka, Eisuke; Furukawa, Masato; Harada, Seiya; Yoshino, Shuji; Seto, Junji; Ikeda, Tetsuya; Yamaguchi, Keiji; Murase, Kazunori; Gotoh, Yasuhiro; Imuta, Naoko; Nishi, Junichiro; Gomes, Tânia A.; Beutin, Lothar; Hayashi, Tetsuya.

In: Genome Biology and Evolution, Vol. 7, No. 12, 01.01.2015, p. 3170-3179.

Research output: Contribution to journalArticle

Ooka, T, Ogura, Y, Katsura, K, Seto, K, Kobayashi, H, Kawano, K, Tokuoka, E, Furukawa, M, Harada, S, Yoshino, S, Seto, J, Ikeda, T, Yamaguchi, K, Murase, K, Gotoh, Y, Imuta, N, Nishi, J, Gomes, TA, Beutin, L & Hayashi, T 2015, 'Defining the genome features of Escherichia albertii, an emerging enteropathogen closely related to Escherichia coli', Genome Biology and Evolution, vol. 7, no. 12, pp. 3170-3179. https://doi.org/10.1093/gbe/evv211
Ooka, Tadasuke ; Ogura, Yoshitoshi ; Katsura, Keisuke ; Seto, Kazuko ; Kobayashi, Hideki ; Kawano, Kimiko ; Tokuoka, Eisuke ; Furukawa, Masato ; Harada, Seiya ; Yoshino, Shuji ; Seto, Junji ; Ikeda, Tetsuya ; Yamaguchi, Keiji ; Murase, Kazunori ; Gotoh, Yasuhiro ; Imuta, Naoko ; Nishi, Junichiro ; Gomes, Tânia A. ; Beutin, Lothar ; Hayashi, Tetsuya. / Defining the genome features of Escherichia albertii, an emerging enteropathogen closely related to Escherichia coli. In: Genome Biology and Evolution. 2015 ; Vol. 7, No. 12. pp. 3170-3179.
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AU - Harada, Seiya

AU - Yoshino, Shuji

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