Defining the immunological phenotype of Fc receptor-like B (FCRLB) deficient mice: Confounding role of the inhibitory FcγRIIb

Keiji Masuda, Hiromi Mori, Osamu Ohara, Manabu Nakayama, Ji Yang Wang, Peter D. Burrows

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Fc receptor-like A (FCRLA) and FCRLB have homology to the transmembrane FCRL family members (FCRL 1-6) and to the conventional receptors for the Fc portion of immunoglobulin, but uniquely are cytosolic proteins expressed in B cells. Here we describe the phenotype of Fcrlb-gene targeted mice. B cell development and in vitro responses are normal; however, antibody responses to a T-dependent antigen are elevated. The gene encoding the inhibitory FcγRIIb is located nearby Fcrlb. Although Fcrlb-gene targeting had no effect on the function or basal expression of FcγRIIb, its expression was reduced following activation. This abnormal regulation was due to co-inheritance of Fcgr2b and the mutant Fcrlb allele from the 129 ES cells. A promoter polymorphism in the 129/Sv Fcgr2b allele results in diminished upregulation of FcγRIIb following B cell activation. Thus, we speculate that the enhanced antibody response seen in the FCRLB-deficient mice may be due to the Fcgr2b promoter.

Original languageEnglish
Pages (from-to)24-31
Number of pages8
JournalCellular Immunology
Volume266
Issue number1
DOIs
Publication statusPublished - Sep 24 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology

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