Degradation of a non-phenolic β-0-4 lignin model dimer by Cerrena unicolor laccase and mediators, acetovanillone and acetosyringone

Nam Seok Cho, Andrzej Leonowicz, Anna Jarosz-Wilkolazka, Grazyna Ginalska, Hee Yeon Cho, Soo Jeong Shin, Yun Jeong Choi, Shoji Ohga

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This study focuses on the laccase and mediator co-operation in modelling lignin dimer veratrylglycerol-β-vanillate ether (WE) oxidation. Earlier we stated that constitutive laccase isolated from Cerrena unicolor can cooperate with acetovanillone (AV) and acetosyringone (AS) in degradation of high molecular fraction of lignosulfonates. The fungus C. unicolor is known as a very good producer of cheap constitutive laccase. Its cultivation for this reason does not require stimulation by the expensive and toxic inducer like 2,5-xylidine. The main purpose of present study is to examine capability of C. unicolor laccase to oxidize VVE, which is a non-phenolic lignin model compound with external mediator HBT, and AV and AS in order to choose one, most efficient system. In the presence of laccase, generation of monomeric aromatic acids from non-phenolic lignin model dimer veratrylglycerol-β-vanillate ether (WE) was observed. The addition of acetovanillone (AV) or acetosyringone (AS) intensified this process, i.e. transformation was more extensive than in the experiments omitting mediators. Among the products, isovanillic (IA) and vanillic (VA) acids were identified.

Original languageEnglish
Pages (from-to)7-12
Number of pages6
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume53
Issue number1
Publication statusPublished - Feb 1 2008

Fingerprint

acetosyringone
Laccase
laccase
Lignin
lignin
degradation
Ether
ethers
Vanillic Acid
aromatic acids
lignosulfonates
vanillic acid
Poisons
Fungi
Cerrena unicolor
acetovanillone
oxidation
fungi

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Cho, N. S., Leonowicz, A., Jarosz-Wilkolazka, A., Ginalska, G., Cho, H. Y., Shin, S. J., ... Ohga, S. (2008). Degradation of a non-phenolic β-0-4 lignin model dimer by Cerrena unicolor laccase and mediators, acetovanillone and acetosyringone. Journal of the Faculty of Agriculture, Kyushu University, 53(1), 7-12.

Degradation of a non-phenolic β-0-4 lignin model dimer by Cerrena unicolor laccase and mediators, acetovanillone and acetosyringone. / Cho, Nam Seok; Leonowicz, Andrzej; Jarosz-Wilkolazka, Anna; Ginalska, Grazyna; Cho, Hee Yeon; Shin, Soo Jeong; Choi, Yun Jeong; Ohga, Shoji.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 53, No. 1, 01.02.2008, p. 7-12.

Research output: Contribution to journalArticle

Cho, NS, Leonowicz, A, Jarosz-Wilkolazka, A, Ginalska, G, Cho, HY, Shin, SJ, Choi, YJ & Ohga, S 2008, 'Degradation of a non-phenolic β-0-4 lignin model dimer by Cerrena unicolor laccase and mediators, acetovanillone and acetosyringone', Journal of the Faculty of Agriculture, Kyushu University, vol. 53, no. 1, pp. 7-12.
Cho, Nam Seok ; Leonowicz, Andrzej ; Jarosz-Wilkolazka, Anna ; Ginalska, Grazyna ; Cho, Hee Yeon ; Shin, Soo Jeong ; Choi, Yun Jeong ; Ohga, Shoji. / Degradation of a non-phenolic β-0-4 lignin model dimer by Cerrena unicolor laccase and mediators, acetovanillone and acetosyringone. In: Journal of the Faculty of Agriculture, Kyushu University. 2008 ; Vol. 53, No. 1. pp. 7-12.
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