Degradation of nuclear DNA by DNase II-like acid DNase in cortical fiber cells of mouse eye lens

Masaki Nakahara, Akiomi Nagasaka, Masato Koike, Kaori Uchida, Kohki Kawane, Yasuo Uchiyama, Shigekazu Nagata

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The eye lens is composed of fiber cells that differentiate from epithelial cells on its anterior surface. In concert with this differentiation, a set of proteins essential for lens function is synthesized, and the cellular organelles are degraded. DNase II-like acid DNase, also called DNase IIβ, is specifically expressed in the lens, and degrades the DNA in the lens fiber cells. Here we report that DNase II-like acid DNase is synthesized as a precursor with a signal sequence, and is localized to lysosomes. DNase II-like acid DNase mRNA was found in cortical fiber cells but not epithelial cells, indicating that its expression is induced during the differentiation of epithelial cells into fiber cells. Immunohistochemical and immunocytochemical analyses indicated that DNase II-like acid DNase was colocalized with Lamp-1 in the lysosomes of fiber cells in a relatively narrow region bordering the organelle-free zone, and was often found in degenerating nuclei. A comparison by microarray analysis of the gene expression profiles between epithelial and cortical fiber cells of young mouse lens indicated that some genes for lysosomal enzymes (cathepsins and lipases) were strongly expressed in the fiber cells. These results suggest that the lysosomal system plays a role in the degradation of cellular organelles during lens cell differentiation.

Original languageEnglish
Pages (from-to)3055-3064
Number of pages10
JournalFEBS Journal
Volume274
Issue number12
DOIs
Publication statusPublished - Jun 1 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry

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