Deletion of Mls‐reactive T cells in H‐2‐compatible but Mls‐incompatible bone marrow chimeras

Yasunobu Yoshikai, Masaro Ogimoto, Koji Matsumoto, Misao Sakumoto, Goro Matsuzaki, Kikuo Nomoto

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    The cellular basis of tolerance induction to Mls‐encoded antigens in radiation bone marrow (BM) chimeras has been investigated in two H‐2‐compatible strain combinations of AKR/J (H‐2k, Thy‐1.1, Mls‐Ia) and C3H/He (H‐2k, Thy‐1.2, Mls‐IIa). Sequential appearance of host‐ and donor‐derived T cell subsets and T cell receptor gene messages occurred in the peripheral lymphoid organs of both irradiated AKR/J mice reconstituted with C3H/He BM cells (C3H/He‐AKR/J chimera) and irradiated C3H/He mice reconstituted with AKR/J BM cells (AKR/J‐C3H/He chimera). A large number of cells expressing T cell receptor γ genes were detected in spleen on day 21 after reconstitution, while the normal level of alloreactivity was first detected in the spleen on day 56 after reconstitution in correlation with the appearance of appreciable levels of Thy‐1high cells and T cell receptor α and β gene transcripts. T cells bearing Vβ6, that is strongly correlated with reactivity to antigens encoded by the Mls‐Ia genetic locus, were virtually abolished in spleen on day 56 in both C3H/He‐AKR/J chimera and AKR/J‐C3H/He chimera. Furthermore, expression of Vβ3 gene transcripts, that are important for recognizing Mls‐IIa, was undetected either in the peripheral lymphoid cells of AKR/J‐C3H/He chimera or in those of C3H/He‐AKR/J chimera. These results suggested that clonal elimination of self‐reactive T cells bearing Vβ3 or Vβ6 was induced by both host‐derived radioresistant cells and donor‐derived repopulating cells in the thymus of radiation BM chimeras.

    Original languageEnglish
    Pages (from-to)1009-1013
    Number of pages5
    JournalEuropean Journal of Immunology
    Volume19
    Issue number6
    DOIs
    Publication statusPublished - Jan 1 1989

    Fingerprint

    Bone Marrow
    T-Lymphocytes
    T-Cell Receptor Genes
    Spleen
    Bone Marrow Cells
    Inbred AKR Mouse
    Radiation
    Antigens
    Genetic Loci
    Inbred C3H Mouse
    T-Lymphocyte Subsets
    Thymus Gland
    Cell Count
    Lymphocytes
    Gene Expression

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Yoshikai, Y., Ogimoto, M., Matsumoto, K., Sakumoto, M., Matsuzaki, G., & Nomoto, K. (1989). Deletion of Mls‐reactive T cells in H‐2‐compatible but Mls‐incompatible bone marrow chimeras. European Journal of Immunology, 19(6), 1009-1013. https://doi.org/10.1002/eji.1830190609

    Deletion of Mls‐reactive T cells in H‐2‐compatible but Mls‐incompatible bone marrow chimeras. / Yoshikai, Yasunobu; Ogimoto, Masaro; Matsumoto, Koji; Sakumoto, Misao; Matsuzaki, Goro; Nomoto, Kikuo.

    In: European Journal of Immunology, Vol. 19, No. 6, 01.01.1989, p. 1009-1013.

    Research output: Contribution to journalArticle

    Yoshikai, Y, Ogimoto, M, Matsumoto, K, Sakumoto, M, Matsuzaki, G & Nomoto, K 1989, 'Deletion of Mls‐reactive T cells in H‐2‐compatible but Mls‐incompatible bone marrow chimeras', European Journal of Immunology, vol. 19, no. 6, pp. 1009-1013. https://doi.org/10.1002/eji.1830190609
    Yoshikai, Yasunobu ; Ogimoto, Masaro ; Matsumoto, Koji ; Sakumoto, Misao ; Matsuzaki, Goro ; Nomoto, Kikuo. / Deletion of Mls‐reactive T cells in H‐2‐compatible but Mls‐incompatible bone marrow chimeras. In: European Journal of Immunology. 1989 ; Vol. 19, No. 6. pp. 1009-1013.
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    abstract = "The cellular basis of tolerance induction to Mls‐encoded antigens in radiation bone marrow (BM) chimeras has been investigated in two H‐2‐compatible strain combinations of AKR/J (H‐2k, Thy‐1.1, Mls‐Ia) and C3H/He (H‐2k, Thy‐1.2, Mls‐IIa). Sequential appearance of host‐ and donor‐derived T cell subsets and T cell receptor gene messages occurred in the peripheral lymphoid organs of both irradiated AKR/J mice reconstituted with C3H/He BM cells (C3H/He‐AKR/J chimera) and irradiated C3H/He mice reconstituted with AKR/J BM cells (AKR/J‐C3H/He chimera). A large number of cells expressing T cell receptor γ genes were detected in spleen on day 21 after reconstitution, while the normal level of alloreactivity was first detected in the spleen on day 56 after reconstitution in correlation with the appearance of appreciable levels of Thy‐1high cells and T cell receptor α and β gene transcripts. T cells bearing Vβ6, that is strongly correlated with reactivity to antigens encoded by the Mls‐Ia genetic locus, were virtually abolished in spleen on day 56 in both C3H/He‐AKR/J chimera and AKR/J‐C3H/He chimera. Furthermore, expression of Vβ3 gene transcripts, that are important for recognizing Mls‐IIa, was undetected either in the peripheral lymphoid cells of AKR/J‐C3H/He chimera or in those of C3H/He‐AKR/J chimera. These results suggested that clonal elimination of self‐reactive T cells bearing Vβ3 or Vβ6 was induced by both host‐derived radioresistant cells and donor‐derived repopulating cells in the thymus of radiation BM chimeras.",
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