Deletion of the gene encoding cytochrome b 562 from Rhodobacter sphaeroides

Chang Hyon Yun, Blanca Barquera, Koh Iba, Ken ichiro Takamiya, James Shapleigh, Antony R. Crofts, Robert B. Gennis

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cytochrome b 562 is a membrane bound di-heme cytochrome previously isolated and characterized from the membranes of photosynthetically grown Rhodobacter sphaeroides. Although originally suggested to be a component of the bc 1 complex, subsequent work, including the sequence of the gene encoding the protein, indicates that this is not the case. More recently, it has been proposed to be a component of complex II (succinate-ubiquinol oxidoreductase). In an effort to determine the function of this cytochrome, the gene encoding the protein has been deleted from the chromosome, and the resulting strain has been examined. The results show that this cytochrome is not as essential component of complex II, the bc 1 complex, or any of the respiratory oxidases.

Original languageEnglish
Pages (from-to)105-110
Number of pages6
JournalFEMS microbiology letters
Volume120
Issue number1-2
DOIs
Publication statusPublished - Jul 1 1994

Fingerprint

Rhodobacter sphaeroides
Cytochromes b
Gene Deletion
Cytochromes
Oxidoreductases
Membranes
Succinic Acid
Heme
Proteins
Chromosomes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Yun, C. H., Barquera, B., Iba, K., Takamiya, K. I., Shapleigh, J., Crofts, A. R., & Gennis, R. B. (1994). Deletion of the gene encoding cytochrome b 562 from Rhodobacter sphaeroides FEMS microbiology letters, 120(1-2), 105-110. https://doi.org/10.1111/j.1574-6968.1994.tb07015.x

Deletion of the gene encoding cytochrome b 562 from Rhodobacter sphaeroides . / Yun, Chang Hyon; Barquera, Blanca; Iba, Koh; Takamiya, Ken ichiro; Shapleigh, James; Crofts, Antony R.; Gennis, Robert B.

In: FEMS microbiology letters, Vol. 120, No. 1-2, 01.07.1994, p. 105-110.

Research output: Contribution to journalArticle

Yun, CH, Barquera, B, Iba, K, Takamiya, KI, Shapleigh, J, Crofts, AR & Gennis, RB 1994, ' Deletion of the gene encoding cytochrome b 562 from Rhodobacter sphaeroides ', FEMS microbiology letters, vol. 120, no. 1-2, pp. 105-110. https://doi.org/10.1111/j.1574-6968.1994.tb07015.x
Yun, Chang Hyon ; Barquera, Blanca ; Iba, Koh ; Takamiya, Ken ichiro ; Shapleigh, James ; Crofts, Antony R. ; Gennis, Robert B. / Deletion of the gene encoding cytochrome b 562 from Rhodobacter sphaeroides In: FEMS microbiology letters. 1994 ; Vol. 120, No. 1-2. pp. 105-110.
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