Delimitation of cryptic species of the Scytosiphon lomentaria complex (Scytosiphonaceae, Phaeophyceae) in Japan, based on mitochondrial and nuclear molecular markers

Kazuhiro Kogame, Shozo Ishikawa, Kei Yamauchi, Shinya Uwai, Akira Kurihara, Michio Masuda

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Scytosiphon lomentaria (Scytosiphonaceae, Ectocarpales) is believed to include some cryptic species, particularly in the Pacific. We attempted to delimit these species in Japan using mitochondrial cox1 and cox3 and nuclear ITS2 and the second intron of the centrin gene (cetn-int2). Fifty-three cox1+cox3 mitotypes, 26 ITS2 ribotypes and 45 cetn-int2 haplotypes were found in 107 samples collected from 33 localities in Japan. Based on phylogenetic analyses, similar sequence types were grouped into ten mitogroups, eight ribogroups and six cetn-int2 haplogroups (sequence-type groups). From the molecular trees and combinations of the mito-, ribo- and haplogroups, three cryptic species were apparent (Groups I-III). Group I, widely distributed on Pacific coasts, was highly supported by all molecular trees, whereas Groups II (North Pacific) and III (Northwestern Pacific and Australasia) were more closely related to each other. However, sequence-type-group combinations that would be characteristic of hybrids between Groups II and III were not detected, suggesting no gene flow between the two Groups. Further investigations of an additional 127 sympatrically growing plants supported the absence of gene flow between Groups II and III. Four samples did not belong to any of the Groups I-III and possibly represent additional species.

Original languageEnglish
Pages (from-to)167-177
Number of pages11
JournalPhycological Research
Volume63
Issue number3
DOIs
Publication statusPublished - Jul 1 2015

Fingerprint

Lomentaria
Phaeophyta
Phaeophyceae
Gene Flow
gene flow
Japan
Ectocarpales
Australasia
Ribotyping
ribotypes
genetic markers
Australasian region
Sulfamethoxazole Drug Combination Trimethoprim
Introns
Haplotypes
Sequence Analysis
introns
haplotypes
coasts
sampling

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Physiology

Cite this

Delimitation of cryptic species of the Scytosiphon lomentaria complex (Scytosiphonaceae, Phaeophyceae) in Japan, based on mitochondrial and nuclear molecular markers. / Kogame, Kazuhiro; Ishikawa, Shozo; Yamauchi, Kei; Uwai, Shinya; Kurihara, Akira; Masuda, Michio.

In: Phycological Research, Vol. 63, No. 3, 01.07.2015, p. 167-177.

Research output: Contribution to journalArticle

@article{d1e762fb252941e0bdeded6864fcfb5e,
title = "Delimitation of cryptic species of the Scytosiphon lomentaria complex (Scytosiphonaceae, Phaeophyceae) in Japan, based on mitochondrial and nuclear molecular markers",
abstract = "Scytosiphon lomentaria (Scytosiphonaceae, Ectocarpales) is believed to include some cryptic species, particularly in the Pacific. We attempted to delimit these species in Japan using mitochondrial cox1 and cox3 and nuclear ITS2 and the second intron of the centrin gene (cetn-int2). Fifty-three cox1+cox3 mitotypes, 26 ITS2 ribotypes and 45 cetn-int2 haplotypes were found in 107 samples collected from 33 localities in Japan. Based on phylogenetic analyses, similar sequence types were grouped into ten mitogroups, eight ribogroups and six cetn-int2 haplogroups (sequence-type groups). From the molecular trees and combinations of the mito-, ribo- and haplogroups, three cryptic species were apparent (Groups I-III). Group I, widely distributed on Pacific coasts, was highly supported by all molecular trees, whereas Groups II (North Pacific) and III (Northwestern Pacific and Australasia) were more closely related to each other. However, sequence-type-group combinations that would be characteristic of hybrids between Groups II and III were not detected, suggesting no gene flow between the two Groups. Further investigations of an additional 127 sympatrically growing plants supported the absence of gene flow between Groups II and III. Four samples did not belong to any of the Groups I-III and possibly represent additional species.",
author = "Kazuhiro Kogame and Shozo Ishikawa and Kei Yamauchi and Shinya Uwai and Akira Kurihara and Michio Masuda",
year = "2015",
month = "7",
day = "1",
doi = "10.1111/pre.12091",
language = "English",
volume = "63",
pages = "167--177",
journal = "Phycological Research",
issn = "1322-0829",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

TY - JOUR

T1 - Delimitation of cryptic species of the Scytosiphon lomentaria complex (Scytosiphonaceae, Phaeophyceae) in Japan, based on mitochondrial and nuclear molecular markers

AU - Kogame, Kazuhiro

AU - Ishikawa, Shozo

AU - Yamauchi, Kei

AU - Uwai, Shinya

AU - Kurihara, Akira

AU - Masuda, Michio

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Scytosiphon lomentaria (Scytosiphonaceae, Ectocarpales) is believed to include some cryptic species, particularly in the Pacific. We attempted to delimit these species in Japan using mitochondrial cox1 and cox3 and nuclear ITS2 and the second intron of the centrin gene (cetn-int2). Fifty-three cox1+cox3 mitotypes, 26 ITS2 ribotypes and 45 cetn-int2 haplotypes were found in 107 samples collected from 33 localities in Japan. Based on phylogenetic analyses, similar sequence types were grouped into ten mitogroups, eight ribogroups and six cetn-int2 haplogroups (sequence-type groups). From the molecular trees and combinations of the mito-, ribo- and haplogroups, three cryptic species were apparent (Groups I-III). Group I, widely distributed on Pacific coasts, was highly supported by all molecular trees, whereas Groups II (North Pacific) and III (Northwestern Pacific and Australasia) were more closely related to each other. However, sequence-type-group combinations that would be characteristic of hybrids between Groups II and III were not detected, suggesting no gene flow between the two Groups. Further investigations of an additional 127 sympatrically growing plants supported the absence of gene flow between Groups II and III. Four samples did not belong to any of the Groups I-III and possibly represent additional species.

AB - Scytosiphon lomentaria (Scytosiphonaceae, Ectocarpales) is believed to include some cryptic species, particularly in the Pacific. We attempted to delimit these species in Japan using mitochondrial cox1 and cox3 and nuclear ITS2 and the second intron of the centrin gene (cetn-int2). Fifty-three cox1+cox3 mitotypes, 26 ITS2 ribotypes and 45 cetn-int2 haplotypes were found in 107 samples collected from 33 localities in Japan. Based on phylogenetic analyses, similar sequence types were grouped into ten mitogroups, eight ribogroups and six cetn-int2 haplogroups (sequence-type groups). From the molecular trees and combinations of the mito-, ribo- and haplogroups, three cryptic species were apparent (Groups I-III). Group I, widely distributed on Pacific coasts, was highly supported by all molecular trees, whereas Groups II (North Pacific) and III (Northwestern Pacific and Australasia) were more closely related to each other. However, sequence-type-group combinations that would be characteristic of hybrids between Groups II and III were not detected, suggesting no gene flow between the two Groups. Further investigations of an additional 127 sympatrically growing plants supported the absence of gene flow between Groups II and III. Four samples did not belong to any of the Groups I-III and possibly represent additional species.

UR - http://www.scopus.com/inward/record.url?scp=84934438124&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84934438124&partnerID=8YFLogxK

U2 - 10.1111/pre.12091

DO - 10.1111/pre.12091

M3 - Article

VL - 63

SP - 167

EP - 177

JO - Phycological Research

JF - Phycological Research

SN - 1322-0829

IS - 3

ER -