Delivery of lectin-labeled membrane to the Trans-golgi network and secretory granules in cultured atrial myocytes

Hiroshi Iida, Y. Shibata

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

To examine whether and how internalized plasma membrane components are routed to the compartment of the biosynthetic-exocytic pathway in cultured atrial myocytes, the plasma membrane labeled with wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) was traced electron microscopically by cytochemical detection of HRP. The WGA-HRP label was internalized via a coated pit-small vesicle pathway and reached vacuoles and endosomes by 3 min. Labeled endosomes comprised vacuoles and tubular elements containing reaction product. By 15 min, similar tubular structures containing reaction product accumulated in the area of the trans-Golgi network (TGN). The labeled TGN consisted of interconnected tubular elements, which often connected to atrial granules containing reaction product. In contrast, neither native HRP not Lucifer Yellow reached Golgi elements or atrial granules. These results suggest that a proportion of the plasma membrane labeled with WGA-HRP is delivered to endosomes, from which tubules might bud off to transfer the tracer molecules to the TGN, where the lectin conjugate and associated membranes are packaged into atrial granules.

Original languageEnglish
Pages (from-to)1885-1892
Number of pages8
JournalJournal of Histochemistry and Cytochemistry
Volume37
Issue number12
DOIs
Publication statusPublished - Jan 1 1989

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trans-Golgi Network
Wheat Germ Agglutinins
Endosomes
Secretory Vesicles
Horseradish Peroxidase
Lectins
Muscle Cells
Cell Membrane
Vacuoles
Membranes
Biosynthetic Pathways
Electrons

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

Cite this

Delivery of lectin-labeled membrane to the Trans-golgi network and secretory granules in cultured atrial myocytes. / Iida, Hiroshi; Shibata, Y.

In: Journal of Histochemistry and Cytochemistry, Vol. 37, No. 12, 01.01.1989, p. 1885-1892.

Research output: Contribution to journalArticle

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