Delta1 family members are involved in filopodial actin formation and neuronal cell migration independent of Notch signaling

Kazuya Sugiyama, Kenji Nishide, Hiromi Matsuo, Sayumi Okigawa, Makoto Okano, Tohru Ishitani, Kunihiro Matsumoto, Motoyuki Itoh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Delta family proteins are transmembrane molecules that bind Notch receptors and activate downstream signaling events in neighboring cells. In addition to serving as Notch ligands, Notch-independent roles for Delta have been suggested but are not fully understood. Here, we demonstrate a previously unrecognized role for Delta in filopodial actin formation. Delta1 and Delta4, but not Delta3, exhibit filopodial protrusive activity, and this activity is independent of Notch signaling. The filopodial activity of Delta1 does not depend on the PDZ-binding domain at the C-terminus; however, the intracellular membrane-proximal region that is anchored to the plasma membrane plays an important role in filopodial activity. We further identified a Notch-independent role of DeltaD in neuronal cell migration in zebrafish. These findings suggest a possible functional link between Notch-independent filopodial activity of Delta and the control of cell motility.

Original languageEnglish
Pages (from-to)118-124
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume398
Issue number1
DOIs
Publication statusPublished - Jul 1 2010

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Cell Movement
Actins
Notch Receptors
PDZ Domains
Intracellular Membranes
Somatostatin-Secreting Cells
Zebrafish
Cell membranes
Cell Membrane
Ligands
Membranes
Molecules
Proteins
delta protein

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Delta1 family members are involved in filopodial actin formation and neuronal cell migration independent of Notch signaling. / Sugiyama, Kazuya; Nishide, Kenji; Matsuo, Hiromi; Okigawa, Sayumi; Okano, Makoto; Ishitani, Tohru; Matsumoto, Kunihiro; Itoh, Motoyuki.

In: Biochemical and Biophysical Research Communications, Vol. 398, No. 1, 01.07.2010, p. 118-124.

Research output: Contribution to journalArticle

Sugiyama, Kazuya ; Nishide, Kenji ; Matsuo, Hiromi ; Okigawa, Sayumi ; Okano, Makoto ; Ishitani, Tohru ; Matsumoto, Kunihiro ; Itoh, Motoyuki. / Delta1 family members are involved in filopodial actin formation and neuronal cell migration independent of Notch signaling. In: Biochemical and Biophysical Research Communications. 2010 ; Vol. 398, No. 1. pp. 118-124.
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AU - Okigawa, Sayumi

AU - Okano, Makoto

AU - Ishitani, Tohru

AU - Matsumoto, Kunihiro

AU - Itoh, Motoyuki

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