TY - JOUR
T1 - Demethylation by 5‐Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice
AU - Araki, Kimi
AU - Miyazaki, Jun‐ichi ‐i
AU - Tsurimoto, Toshiki
AU - Inomoto, Takeaki
AU - Iwanaga, Tomohisa
AU - Matsubara, Kenichi
AU - Yamamura, Ken‐ichi ‐i
PY - 1989/4
Y1 - 1989/4
N2 - In 14p3HB transgenic mice, which carry three tandem copies of hepatitis B virus (HBV) DNA, the HBV DNA was significantly methylated and no viral proteins were produced. To analyze the causal relationship between hypermethylation and gene inactivity, 5‐azacytidine was injected into the mice to demethylate HBV DNA. When postnatal 14p3HB mice were treated with the drug, hepatitis virus surface antigen was produced in these mice by 3 weeks of age, and the integrated HBV DNA of the liver was less heavily methylated. Our results suggest that injection of 5‐azacytidine can be used to efficiently activate a silent transgene such as HBV DNA in transgenic mice.
AB - In 14p3HB transgenic mice, which carry three tandem copies of hepatitis B virus (HBV) DNA, the HBV DNA was significantly methylated and no viral proteins were produced. To analyze the causal relationship between hypermethylation and gene inactivity, 5‐azacytidine was injected into the mice to demethylate HBV DNA. When postnatal 14p3HB mice were treated with the drug, hepatitis virus surface antigen was produced in these mice by 3 weeks of age, and the integrated HBV DNA of the liver was less heavily methylated. Our results suggest that injection of 5‐azacytidine can be used to efficiently activate a silent transgene such as HBV DNA in transgenic mice.
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U2 - 10.1111/j.1349-7006.1989.tb02308.x
DO - 10.1111/j.1349-7006.1989.tb02308.x
M3 - Article
C2 - 2473052
AN - SCOPUS:0024797059
SN - 1347-9032
VL - 80
SP - 295
EP - 298
JO - Cancer Science
JF - Cancer Science
IS - 4
ER -
