Demethylation by 5‐Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice

Kimi Araki, Jun‐ichi ‐i Miyazaki, Toshiki Tsurimoto, Takeaki Inomoto, Tomohisa Iwanaga, Kenichi Matsubara, Ken‐ichi ‐i Yamamura

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In 14p3HB transgenic mice, which carry three tandem copies of hepatitis B virus (HBV) DNA, the HBV DNA was significantly methylated and no viral proteins were produced. To analyze the causal relationship between hypermethylation and gene inactivity, 5‐azacytidine was injected into the mice to demethylate HBV DNA. When postnatal 14p3HB mice were treated with the drug, hepatitis virus surface antigen was produced in these mice by 3 weeks of age, and the integrated HBV DNA of the liver was less heavily methylated. Our results suggest that injection of 5‐azacytidine can be used to efficiently activate a silent transgene such as HBV DNA in transgenic mice.

Original languageEnglish
Pages (from-to)295-298
Number of pages4
JournalJapanese Journal of Cancer Research
Volume80
Issue number4
DOIs
Publication statusPublished - Apr 1989
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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