Denaturing gradient gel electrophoresis analysis of gut bacterial community for the Japanese earthworms

Kazunori Nakamura, Kenji Sakai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The gut bacterial community structure for Pheretima hilgendorfi and P. heteropoda (Family Megascolecidae), and Allolobophora japonica (Family Lumbricidae) collected from agricultural grasslands in Japan was analyzed by denaturing gradient gel electrophoresis of polymerase chain reaction-amplified 16S rRNA gene fragments (PCR-DGGE) and compared with those in the surrounding soils. Denaturing gradient gel electrophoresis (DGGE) profiles indicated that each earthworm species had their own specific bacterial communities, and multidimentional scaling analysis grouped the DGGE profiles into three groups: gut samples from P. hilgendorfi and P. heteropoda, gut samples from A. japonica and samples from the surrounding soils. Nine dominant bands were identified by their direct sequencing and cloning. Major three bands from P. hilgendorfi and P. heteropoda were closely related to Bacillus species belonging to the phylum Firmicutes. Major four and two bands from A. japonica were closely related to the phyla Proteobacteria and Bacteroidetes, respectively.

Original languageEnglish
Pages (from-to)519-528
Number of pages10
JournalSoil Science and Plant Nutrition
Volume57
Issue number4
DOIs
Publication statusPublished - Dec 1 2011

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Heteropoda
denaturing gradient gel electrophoresis
earthworms
bacterial communities
earthworm
electrokinesis
gel
digestive system
Allolobophora
Pheretima
Megascolecidae
Lumbricidae
Firmicutes
Proteobacteria
sampling
polymerase chain reaction
molecular cloning
soil
community structure
grasslands

All Science Journal Classification (ASJC) codes

  • Soil Science
  • Plant Science

Cite this

Denaturing gradient gel electrophoresis analysis of gut bacterial community for the Japanese earthworms. / Nakamura, Kazunori; Sakai, Kenji.

In: Soil Science and Plant Nutrition, Vol. 57, No. 4, 01.12.2011, p. 519-528.

Research output: Contribution to journalArticle

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