Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis

Sho Hei Uchi, Ryoji Yanai, Masaaki Kobayashi, Makoto Hatano, Yuka Kobayashi, Chiemi Yamashiro, Tomohiko Nagai, Kazuo Tokuda, Kip M. Connor, Kohei Sonoda, Kazuhiro Kimura

Research output: Contribution to journalArticle

Abstract

We previously showed that dietary omega (ω)–3 long-chain polyunsaturated fatty acids (LCPUFAs) suppress inflammation in mice with experimental autoimmune uveitis (EAU). We have now investigated the role of antigen presenting cells (APCs) in this action of ω-3 LCPUFAs. C57BL/6 mice were fed a diet supplemented with ω-3 or ω-6 LCPUFAs for 2 weeks, after which splenocytes were isolated from the mice and cocultured with CD4+ T cells isolated from mice with EAU induced by injection of a human interphotoreceptor retinoid-binding protein peptide together with complete Freund’s adjuvant. The proliferation of and production of interferon-γ and interleukin-17 by T cells from EAU mice in vitro were attenuated in the presence of splenocytes from ω-3 LCPUFA–fed mice as compared with those from mice fed ω-6 LCPUFAs. Splenocyte fractionation by magnetic-activated cell sorting revealed that, among APCs, dendritic cells (DCs) were the target of ω-3 LCPUFAs. Adoptive transfer of DCs from mice fed ω-3 LCPUFAs attenuated disease progression in EAU mice as well as the production of pro-inflammatory cytokines by T cells isolated from these latter animals. The proliferation of T cells from control Balb/c mice was also attenuated in the presence of DCs from ω-3 LCPUFA–fed mice as compared with those from ω-6 LCPUFA–fed mice. Furthermore, T cell proliferation in such a mixed lymphocyte reaction was inhibited by prior exposure of DCs from mice fed an ω-6 LCPUFA diet to ω-3 LCPUFAs in vitro. Our results thus suggest that DCs mediate the anti-inflammatory action of dietary ω-3 LCPUFAs in EAU.

Original languageEnglish
Article numbere0219405
JournalPloS one
Volume14
Issue number7
DOIs
Publication statusPublished - Jan 1 2019

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long chain polyunsaturated fatty acids
Uveitis
dendritic cells
Unsaturated Fatty Acids
Dendritic Cells
Anti-Inflammatory Agents
T-cells
mice
T-lymphocytes
T-Lymphocytes
splenocytes
Nutrition
antigen-presenting cells
Antigen-Presenting Cells
Lymphocytes
Interleukin-17
Freund's Adjuvant
Cell proliferation
Fractionation
Diet

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Uchi, S. H., Yanai, R., Kobayashi, M., Hatano, M., Kobayashi, Y., Yamashiro, C., ... Kimura, K. (2019). Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis. PloS one, 14(7), [e0219405]. https://doi.org/10.1371/journal.pone.0219405

Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis. / Uchi, Sho Hei; Yanai, Ryoji; Kobayashi, Masaaki; Hatano, Makoto; Kobayashi, Yuka; Yamashiro, Chiemi; Nagai, Tomohiko; Tokuda, Kazuo; Connor, Kip M.; Sonoda, Kohei; Kimura, Kazuhiro.

In: PloS one, Vol. 14, No. 7, e0219405, 01.01.2019.

Research output: Contribution to journalArticle

Uchi, SH, Yanai, R, Kobayashi, M, Hatano, M, Kobayashi, Y, Yamashiro, C, Nagai, T, Tokuda, K, Connor, KM, Sonoda, K & Kimura, K 2019, 'Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis', PloS one, vol. 14, no. 7, e0219405. https://doi.org/10.1371/journal.pone.0219405
Uchi, Sho Hei ; Yanai, Ryoji ; Kobayashi, Masaaki ; Hatano, Makoto ; Kobayashi, Yuka ; Yamashiro, Chiemi ; Nagai, Tomohiko ; Tokuda, Kazuo ; Connor, Kip M. ; Sonoda, Kohei ; Kimura, Kazuhiro. / Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis. In: PloS one. 2019 ; Vol. 14, No. 7.
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abstract = "We previously showed that dietary omega (ω)–3 long-chain polyunsaturated fatty acids (LCPUFAs) suppress inflammation in mice with experimental autoimmune uveitis (EAU). We have now investigated the role of antigen presenting cells (APCs) in this action of ω-3 LCPUFAs. C57BL/6 mice were fed a diet supplemented with ω-3 or ω-6 LCPUFAs for 2 weeks, after which splenocytes were isolated from the mice and cocultured with CD4+ T cells isolated from mice with EAU induced by injection of a human interphotoreceptor retinoid-binding protein peptide together with complete Freund’s adjuvant. The proliferation of and production of interferon-γ and interleukin-17 by T cells from EAU mice in vitro were attenuated in the presence of splenocytes from ω-3 LCPUFA–fed mice as compared with those from mice fed ω-6 LCPUFAs. Splenocyte fractionation by magnetic-activated cell sorting revealed that, among APCs, dendritic cells (DCs) were the target of ω-3 LCPUFAs. Adoptive transfer of DCs from mice fed ω-3 LCPUFAs attenuated disease progression in EAU mice as well as the production of pro-inflammatory cytokines by T cells isolated from these latter animals. The proliferation of T cells from control Balb/c mice was also attenuated in the presence of DCs from ω-3 LCPUFA–fed mice as compared with those from ω-6 LCPUFA–fed mice. Furthermore, T cell proliferation in such a mixed lymphocyte reaction was inhibited by prior exposure of DCs from mice fed an ω-6 LCPUFA diet to ω-3 LCPUFAs in vitro. Our results thus suggest that DCs mediate the anti-inflammatory action of dietary ω-3 LCPUFAs in EAU.",
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