Dendritic sugar-microarrays by click chemistry

Tomohiro Fukuda, Shunsuke Onogi, Yoshiko Miura

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Saccharide microarrays with well-defined multivalency were prepared using glycodendrimers. The glycodendrimers with various generations and saccharides (α-Man, β-GlcNAc and β-Gal) were synthesized and immobilized on an acetylenyl-terminated gold substrate via click chemistry. The saccharide-immobilized substrates were analyzed using XPS, ellipsometry, cyclic voltammetry, contact angle goniometry, QCM and FTIR-RAS. The biological abilities of the saccharide-immobilized substrates were evaluated by SPR with the lectins of Con A, WGA and RCA120. Lectin binding to the glycodendrimer array was quantitatively analyzed, and association constants (KA) and kinetic rate constants (ka and kd) were evaluated. These kinetic constants were unique to each lectin, implying specificity of the multivalent effect of saccharide-lectin interaction.

Original languageEnglish
Pages (from-to)880-888
Number of pages9
JournalThin Solid Films
Volume518
Issue number2
DOIs
Publication statusPublished - Nov 30 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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