Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization

Shigeki Suzuki, Taduru Sreenath, Naoto Haruyama, Cherlita Honeycutt, Anita Terse, Andrew Cho, Thomas Kohler, Ralph Müller, Michel Goldberg, Ashok B. Kulkarni

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Dentin sialophosphoprotein (DSPP), a major non-collagenous matrix protein of odontoblasts, is proteolytically cleaved into dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). Our previous studies revealed that DSPP null mice display a phenotype similar to human autosomal dominant dentinogenesis imperfecta, in which teeth have widened predentin and irregular dentin mineralization resulting in sporadic unmineralized areas in dentin and frequent pulp exposure. Earlier in vitro studies suggested that DPP, but not DSP, plays a significant role in initiation and maturation of dentin mineralization. However, the precise in vivo roles of DSP and DPP are far from clear. Here we report the generation of DPPcKO mice, in which only DSP is expressed in a DSPP null background, resulting in a conditional DPP knockout. DPPcKO teeth show a partial rescue of the DSPP null phenotype with the restored predentin width, an absence of irregular unmineralized areas in dentin, and less frequent pulp exposure. Micro-computed tomography (micro-CT) analysis of DPPcKO molars further confirmed this partial rescue with a significant recovery in the dentin volume, but not in the dentin mineral density. These results indicate distinct roles of DSP and DPP in dentin mineralization, with DSP regulating initiation of dentin mineralization, and DPP being involved in the maturation of mineralized dentin.

Original languageEnglish
Pages (from-to)221-229
Number of pages9
JournalMatrix Biology
Volume28
Issue number4
DOIs
Publication statusPublished - May 1 2009
Externally publishedYes

Fingerprint

Dentin
dentin sialophosphoprotein
Tooth
Dentinogenesis Imperfecta
Odontoblasts
Phenotype
Minerals

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Suzuki, S., Sreenath, T., Haruyama, N., Honeycutt, C., Terse, A., Cho, A., ... Kulkarni, A. B. (2009). Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization. Matrix Biology, 28(4), 221-229. https://doi.org/10.1016/j.matbio.2009.03.006

Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization. / Suzuki, Shigeki; Sreenath, Taduru; Haruyama, Naoto; Honeycutt, Cherlita; Terse, Anita; Cho, Andrew; Kohler, Thomas; Müller, Ralph; Goldberg, Michel; Kulkarni, Ashok B.

In: Matrix Biology, Vol. 28, No. 4, 01.05.2009, p. 221-229.

Research output: Contribution to journalArticle

Suzuki, S, Sreenath, T, Haruyama, N, Honeycutt, C, Terse, A, Cho, A, Kohler, T, Müller, R, Goldberg, M & Kulkarni, AB 2009, 'Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization', Matrix Biology, vol. 28, no. 4, pp. 221-229. https://doi.org/10.1016/j.matbio.2009.03.006
Suzuki, Shigeki ; Sreenath, Taduru ; Haruyama, Naoto ; Honeycutt, Cherlita ; Terse, Anita ; Cho, Andrew ; Kohler, Thomas ; Müller, Ralph ; Goldberg, Michel ; Kulkarni, Ashok B. / Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization. In: Matrix Biology. 2009 ; Vol. 28, No. 4. pp. 221-229.
@article{0e66422ad49a445a877a69090baf4440,
title = "Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization",
abstract = "Dentin sialophosphoprotein (DSPP), a major non-collagenous matrix protein of odontoblasts, is proteolytically cleaved into dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). Our previous studies revealed that DSPP null mice display a phenotype similar to human autosomal dominant dentinogenesis imperfecta, in which teeth have widened predentin and irregular dentin mineralization resulting in sporadic unmineralized areas in dentin and frequent pulp exposure. Earlier in vitro studies suggested that DPP, but not DSP, plays a significant role in initiation and maturation of dentin mineralization. However, the precise in vivo roles of DSP and DPP are far from clear. Here we report the generation of DPPcKO mice, in which only DSP is expressed in a DSPP null background, resulting in a conditional DPP knockout. DPPcKO teeth show a partial rescue of the DSPP null phenotype with the restored predentin width, an absence of irregular unmineralized areas in dentin, and less frequent pulp exposure. Micro-computed tomography (micro-CT) analysis of DPPcKO molars further confirmed this partial rescue with a significant recovery in the dentin volume, but not in the dentin mineral density. These results indicate distinct roles of DSP and DPP in dentin mineralization, with DSP regulating initiation of dentin mineralization, and DPP being involved in the maturation of mineralized dentin.",
author = "Shigeki Suzuki and Taduru Sreenath and Naoto Haruyama and Cherlita Honeycutt and Anita Terse and Andrew Cho and Thomas Kohler and Ralph M{\"u}ller and Michel Goldberg and Kulkarni, {Ashok B.}",
year = "2009",
month = "5",
day = "1",
doi = "10.1016/j.matbio.2009.03.006",
language = "English",
volume = "28",
pages = "221--229",
journal = "Matrix Biology",
issn = "0945-053X",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization

AU - Suzuki, Shigeki

AU - Sreenath, Taduru

AU - Haruyama, Naoto

AU - Honeycutt, Cherlita

AU - Terse, Anita

AU - Cho, Andrew

AU - Kohler, Thomas

AU - Müller, Ralph

AU - Goldberg, Michel

AU - Kulkarni, Ashok B.

PY - 2009/5/1

Y1 - 2009/5/1

N2 - Dentin sialophosphoprotein (DSPP), a major non-collagenous matrix protein of odontoblasts, is proteolytically cleaved into dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). Our previous studies revealed that DSPP null mice display a phenotype similar to human autosomal dominant dentinogenesis imperfecta, in which teeth have widened predentin and irregular dentin mineralization resulting in sporadic unmineralized areas in dentin and frequent pulp exposure. Earlier in vitro studies suggested that DPP, but not DSP, plays a significant role in initiation and maturation of dentin mineralization. However, the precise in vivo roles of DSP and DPP are far from clear. Here we report the generation of DPPcKO mice, in which only DSP is expressed in a DSPP null background, resulting in a conditional DPP knockout. DPPcKO teeth show a partial rescue of the DSPP null phenotype with the restored predentin width, an absence of irregular unmineralized areas in dentin, and less frequent pulp exposure. Micro-computed tomography (micro-CT) analysis of DPPcKO molars further confirmed this partial rescue with a significant recovery in the dentin volume, but not in the dentin mineral density. These results indicate distinct roles of DSP and DPP in dentin mineralization, with DSP regulating initiation of dentin mineralization, and DPP being involved in the maturation of mineralized dentin.

AB - Dentin sialophosphoprotein (DSPP), a major non-collagenous matrix protein of odontoblasts, is proteolytically cleaved into dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). Our previous studies revealed that DSPP null mice display a phenotype similar to human autosomal dominant dentinogenesis imperfecta, in which teeth have widened predentin and irregular dentin mineralization resulting in sporadic unmineralized areas in dentin and frequent pulp exposure. Earlier in vitro studies suggested that DPP, but not DSP, plays a significant role in initiation and maturation of dentin mineralization. However, the precise in vivo roles of DSP and DPP are far from clear. Here we report the generation of DPPcKO mice, in which only DSP is expressed in a DSPP null background, resulting in a conditional DPP knockout. DPPcKO teeth show a partial rescue of the DSPP null phenotype with the restored predentin width, an absence of irregular unmineralized areas in dentin, and less frequent pulp exposure. Micro-computed tomography (micro-CT) analysis of DPPcKO molars further confirmed this partial rescue with a significant recovery in the dentin volume, but not in the dentin mineral density. These results indicate distinct roles of DSP and DPP in dentin mineralization, with DSP regulating initiation of dentin mineralization, and DPP being involved in the maturation of mineralized dentin.

UR - http://www.scopus.com/inward/record.url?scp=67349152303&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349152303&partnerID=8YFLogxK

U2 - 10.1016/j.matbio.2009.03.006

DO - 10.1016/j.matbio.2009.03.006

M3 - Article

C2 - 19348940

AN - SCOPUS:67349152303

VL - 28

SP - 221

EP - 229

JO - Matrix Biology

JF - Matrix Biology

SN - 0945-053X

IS - 4

ER -