Depressor effect of wheat germ hydrolysate and its novel angiotensin I- converting enzyme inhibitory peptide, Ile-Val-Tyr, and the metabolism in rat and human plasma

Toshiro Matsui, Chun Hui Li, Toshiaki Tanaka, Tetsuyoshi Maki, Yutaka Osajima, Kiyoshi Matsumoto

Research output: Contribution to journalArticle

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Abstract

We report here the antihypertensive effect of wheat germ (WG) hydrolysate and its dominant peptide, Ile-Val-Tyr (IVY), with potent angiotensin I-converting enzyme (ACE) inhibitory activity. The toxicity test of AG50W fraction purified from the WG hydrolysate and IVY in tidy mice revealed that 1 week median lethal concentrations of AG fraction and IVY were less than 100 and 10 mg/kg, respectively. As a result of an intravenous administration test of both inhibitors in spontaneously hypertensive rat (SHR), the mean arterial blood pressure (MAP) significantly decreased with the dose; the MAP reduction of 10.3 and 19.2 mmHg was observed at a dose of 50 mg/kg of AG fraction and 5 mg/kg of IVY, respectively. In addition to this behavior, the MAP gradually decreased after the 5 mg/kg of IVY injection (time to give a maximum reduction; 8 min), and the reduction was held for 20 min. By using rat and human plasma, IVY was found to be metabolized by the action of aminopeptidase in plasma to form a subsequent ACE inhibitor, Val- Tyr (VY). Thus, the intake of IVY as a physiologically functional food would serve in the lowering of blood pressure (BP) by the combined depressor effect of itself and its metabolite after the absorption.

Original languageEnglish
Pages (from-to)427-431
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume23
Issue number4
DOIs
Publication statusPublished - Jan 1 2000

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isoleucyl-valyl-tyrosine
Peptidyl-Dipeptidase A
Triticum
Arterial Pressure
Peptides
valyltyrosine
Toxicity Tests
Functional Food
Aminopeptidases
Inbred SHR Rats
Angiotensin-Converting Enzyme Inhibitors
Intravenous Administration
Antihypertensive Agents

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Depressor effect of wheat germ hydrolysate and its novel angiotensin I- converting enzyme inhibitory peptide, Ile-Val-Tyr, and the metabolism in rat and human plasma. / Matsui, Toshiro; Li, Chun Hui; Tanaka, Toshiaki; Maki, Tetsuyoshi; Osajima, Yutaka; Matsumoto, Kiyoshi.

In: Biological and Pharmaceutical Bulletin, Vol. 23, No. 4, 01.01.2000, p. 427-431.

Research output: Contribution to journalArticle

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