Depth and time-dependent heterogeneity of microglia in mouse hippocampal slice cultures

Yuka Kasahara, Ryuta Koyama, Yuji Ikegaya

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Microglia are the brain-resident immune cells with the phagocytic capacity to engulf dead and living neurons in health and disease. However, the mechanisms underlying the neuron-microglia interaction remain elusive partly because proper in vitro systems are lacking. Specifically, the highly activated status of microglia with amoeboid morphology in primary culture is different from the ‘resting’ microglia with multiple processes in vivo. Here, we performed a detailed investigation of microglial properties in mouse hippocampal slice cultures, focusing on the changes in morphology in the activated state, finding a depth and time-dependent localization of in vivo-like microglia in slice cultures.

Original languageEnglish
Pages (from-to)64-69
Number of pages6
JournalNeuroscience Research
Volume111
DOIs
Publication statusPublished - Oct 1 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Fingerprint

Dive into the research topics of 'Depth and time-dependent heterogeneity of microglia in mouse hippocampal slice cultures'. Together they form a unique fingerprint.

Cite this