TY - JOUR
T1 - Description of a novel marine bacterium, Vibrio hyugaensis sp. nov., based on genomic and phenotypic characterization
AU - Urbanczyk, Yoshiko
AU - Ogura, Yoshitoshi
AU - Hayashi, Tetsuya
AU - Urbanczyk, Henryk
N1 - Funding Information:
This work was supported by the Program to Disseminate Tenure Tracking System from the Japanese; by a grant for Scientific Research on Priority Areas from the University of Miyazaki ; as well as Kurita Water and Environment Foundation Grant.
Publisher Copyright:
© 2015 Elsevier GmbH.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Three luminous bacteria strains have been isolated from seawater samples collected in the coastal regions of the Miyazaki prefecture in Japan. Analysis of the 16S rRNA gene sequences identified the three strains as members of the genus Vibrio (Vibrionaceae, Gammaproteobacteria), closely related to bacteria in the so-called 'Harveyi clade.' The genomes of the three strains were estimated to be between 5.49Mbp and 5.95Mbp, with average G+C of 43.91%. The genome sequence data was used to estimate relatedness of the three strains to related Vibrio bacteria, including estimation of frequency of recombination events, calculation of average nucleotide identity (ANI), and a phylogenetic analysis based on concatenated alignment of nucleotide sequences of 135 protein coding genes. Results of these analyses in all cases showed the three strains forming a group clearly separate from previously described Vibrio species. A phenotypic analysis revealed that the three strains have character similar to Vibrio bacteria in the 'Harveyi clade', but can be differentiated from previously described species by testing for hydrolysis of esculin. Based on results of genomic, phylogenetic and phenotypic analyses presented in this study, it can be concluded that the three strains represent a novel species, for which the name Vibrio hyugaensis sp. nov. is proposed. The type strain is 090810aT (=LMG 28466T=NBRC 110633T).
AB - Three luminous bacteria strains have been isolated from seawater samples collected in the coastal regions of the Miyazaki prefecture in Japan. Analysis of the 16S rRNA gene sequences identified the three strains as members of the genus Vibrio (Vibrionaceae, Gammaproteobacteria), closely related to bacteria in the so-called 'Harveyi clade.' The genomes of the three strains were estimated to be between 5.49Mbp and 5.95Mbp, with average G+C of 43.91%. The genome sequence data was used to estimate relatedness of the three strains to related Vibrio bacteria, including estimation of frequency of recombination events, calculation of average nucleotide identity (ANI), and a phylogenetic analysis based on concatenated alignment of nucleotide sequences of 135 protein coding genes. Results of these analyses in all cases showed the three strains forming a group clearly separate from previously described Vibrio species. A phenotypic analysis revealed that the three strains have character similar to Vibrio bacteria in the 'Harveyi clade', but can be differentiated from previously described species by testing for hydrolysis of esculin. Based on results of genomic, phylogenetic and phenotypic analyses presented in this study, it can be concluded that the three strains represent a novel species, for which the name Vibrio hyugaensis sp. nov. is proposed. The type strain is 090810aT (=LMG 28466T=NBRC 110633T).
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U2 - 10.1016/j.syapm.2015.04.001
DO - 10.1016/j.syapm.2015.04.001
M3 - Article
C2 - 25952324
AN - SCOPUS:84983126698
SN - 0723-2020
VL - 38
SP - 300
EP - 304
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 5
ER -
