Design and test of filter of high gradient magnetic separation system for trapping immunoglobulin in serum

Hiroshi Ueda, Koh Agatsuma, Kazuhiro Kajikawa, Mitsuho Furuse, Shuichiro Fuchino, Atsushi Ishiyama

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Recently, affinity magnetic beads have been widely used in immunomagnetic cell sorting (IMCS) technology. Today, we can easily sort and analyze DNA and antibodies (immunoglob- ulin) using various types of affinity magnetic beads available in the market. The diameters of these affinity magnetic beads used in immunomagnetic cell sorting are limited to above approximately 1 /xm because of the low magnetic fields induced by permanent magnets. Now, nano-sized affinity magnetic beads are strongly desired to achieve high resolutions. We have been studying and attempting to develop a high-gradient magnetic separation (HGMS) system that employs a superconducting magnet to induce a considerably higher magnetic field than that induced by a permanent magnet in order to trap smaller nano-sized affinity magnetic beads by a filter made of fine stainless steel wool. In this study, we constructed a prototype of a desktop-type HGMS system using a cryocooler-cooled LTS magnet and conducted preliminary experiments on trapping the nano-sized magnetic particles. Furthermore, we investigated the magnetic field distribution and magnetic force around a magnetic wire in the filter by means of a numerical simulation.

Original languageEnglish
Article number5109581
Pages (from-to)2157-2161
Number of pages5
JournalIEEE Transactions on Applied Superconductivity
Volume19
Issue number3
DOIs
Publication statusPublished - Jun 1 2009

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

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