Design of a binuclear Ni(II)-iminodiacetic acid (IDA) complex for selective recognition and covalent labeling of His-tag fused proteins

Ikuko Takahira, Hirokazu Fuchida, Shigekazu Tabata, Naoya Shindo, Shohei Uchinomiya, Itaru Hamachi, Akio Ojida

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Selective protein labeling with a small molecular probe is a versatile method for elucidating protein functions under live-cell conditions. In this Letter, we report the design of the binuclear Ni(II)-iminodiacetic acid (IDA) complex for selective recognition and covalent labeling of His-tag-fused proteins. We found that the Ni(II)-IDA complex 1-2Ni(II) binds to the His6-tag (HHHHHH) with a strong binding affinity (Kd = 24 nM), the value of which is 16-fold higher than the conventional Ni(II)-NTA complex (Kd = 390 nM). The strong binding affinity of the Ni(II)-IDA complex was successfully used in the covalent labeling and fluorescence bioimaging of a His-tag fused GPCR (G-protein coupled receptor) located on the surface of living cells.

Original languageEnglish
Pages (from-to)2855-2858
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume24
Issue number13
DOIs
Publication statusPublished - Jul 1 2014

Fingerprint

His-His-His-His-His-His
Labeling
Molecular Probes
Proteins
G-Protein-Coupled Receptors
Fluorescence
Cells
iminodiacetic acid

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Molecular Medicine
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Medicine(all)

Cite this

Design of a binuclear Ni(II)-iminodiacetic acid (IDA) complex for selective recognition and covalent labeling of His-tag fused proteins. / Takahira, Ikuko; Fuchida, Hirokazu; Tabata, Shigekazu; Shindo, Naoya; Uchinomiya, Shohei; Hamachi, Itaru; Ojida, Akio.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 24, No. 13, 01.07.2014, p. 2855-2858.

Research output: Contribution to journalArticle

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AU - Ojida, Akio

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