Design of a heterotrimeric α-helical bundle by hydrophobic core engineering

Ayumi Kashiwada, Hidekazu Hiroaki, Daisuke Kohda, Mamoru Nango, Toshiki Tanaka

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We previously prepared the de novo designed peptide, [YGG(IEKKIEA)4], which forms a parallel triple-stranded coiled coil. To prepare an AAB-type heterotrimeric α-helical bundle, two variants, where the Ile15 residue in the hydrophobic position was replaced with either an Ala or Trp residue, were designed and named IZ-2A and IZ-2W, respectively. Circular dichroism spectroscopy, peptide titration, sedimentation equilibrium, and gel filtration analyses revealed the formation of an (IZ-2A)2/IZ-2W complex. The NOESY spectra analyses indicated the presence of interstrand interactions between the two Ala residues and the Trp residue in the hydrophobic core. The (IZ-2A)2/IZ-2W complex has the structural uniqueness of native proteins.

Original languageEnglish
Pages (from-to)212-215
Number of pages4
JournalJournal of the American Chemical Society
Volume122
Issue number2
DOIs
Publication statusPublished - Jan 19 2000
Externally publishedYes

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Peptides
Spectrum Analysis
Circular dichroism spectroscopy
Circular Dichroism
Titration
Sedimentation
Gel Chromatography
Gels
Proteins

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Design of a heterotrimeric α-helical bundle by hydrophobic core engineering. / Kashiwada, Ayumi; Hiroaki, Hidekazu; Kohda, Daisuke; Nango, Mamoru; Tanaka, Toshiki.

In: Journal of the American Chemical Society, Vol. 122, No. 2, 19.01.2000, p. 212-215.

Research output: Contribution to journalArticle

Kashiwada, Ayumi ; Hiroaki, Hidekazu ; Kohda, Daisuke ; Nango, Mamoru ; Tanaka, Toshiki. / Design of a heterotrimeric α-helical bundle by hydrophobic core engineering. In: Journal of the American Chemical Society. 2000 ; Vol. 122, No. 2. pp. 212-215.
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