A semi-confocal fluorescence microscope is designed and constructed aiming to observe fluorescence excitation spectrum of soluble molecules adsorbed at the air/water interface. With a conventional confocal fluorescence microscope, the target molecules of pyrene arises some difficulties: excitation wavelength in deep ultraviolet forces low transmission performance of microscopic objectives and low wavelength-tunable performance of an excitation laser. A total internal reflection illumination with a light-guide coupled monochromated-xenon lamp is combined with the confocal fluorescence microscope. Theoretical feasibility and performance of the microscope are discussed with preliminary results obtained.
|Number of pages||4|
|Journal||Evergreen : joint journal of Novel Carbon Resource Sciences & Green Asia Strategy|
|Publication status||Published - Oct 1 2015|
Yang, H., & Harata, A. (2015). Design of a Semi-confocal Fluorescence Microscope for Observing Excitation Spectrum of Soluble Molecules Adsorbed at the Air/water Interface. Evergreen : joint journal of Novel Carbon Resource Sciences & Green Asia Strategy, 2(2), 1-4. http://id.ndl.go.jp/bib/026833788