Design of a Semi-confocal Fluorescence Microscope for Observing Excitation Spectrum of Soluble Molecules Adsorbed at the Air/water Interface

Haiya Yang, Akira Harata

    Research output: Contribution to journalArticle

    Abstract

    A semi-confocal fluorescence microscope is designed and constructed aiming to observe fluorescence excitation spectrum of soluble molecules adsorbed at the air/water interface. With a conventional confocal fluorescence microscope, the target molecules of pyrene arises some difficulties: excitation wavelength in deep ultraviolet forces low transmission performance of microscopic objectives and low wavelength-tunable performance of an excitation laser. A total internal reflection illumination with a light-guide coupled monochromated-xenon lamp is combined with the confocal fluorescence microscope. Theoretical feasibility and performance of the microscope are discussed with preliminary results obtained.
    Original languageEnglish
    Pages (from-to)1-4
    Number of pages4
    JournalEvergreen : joint journal of Novel Carbon Resource Sciences & Green Asia Strategy
    Volume2
    Issue number2
    Publication statusPublished - Oct 1 2015

    Fingerprint Dive into the research topics of 'Design of a Semi-confocal Fluorescence Microscope for Observing Excitation Spectrum of Soluble Molecules Adsorbed at the Air/water Interface'. Together they form a unique fingerprint.

  • Cite this