Design of a semi-confocal fluorescence microscope for observing excitation spectrum of soluble molecules adsorbed at the air/water interface

Haiya Yang, Akira Harata

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    A semi-confocal fluorescence microscope is designed and constructed aiming to observe fluorescence excitation spectrum of soluble molecules adsorbed at the air/water interface. With a conventional confocal fluorescence microscope, the target molecules of pyrene arises some difficulties: excitation wavelength in deep ultraviolet forces low transmission performance of microscopic objectives and low wavelength-tunable performance of an excitation laser. A total internal reflection illumination with a light-guide coupled monochromated-xenon lamp is combined with the confocal fluorescence microscope. Theoretical feasibility and performance of the microscope are discussed with preliminary results obtained.

    Original languageEnglish
    Pages (from-to)1-4
    Number of pages4
    JournalEvergreen
    Volume2
    Issue number2
    DOIs
    Publication statusPublished - Jan 1 2015

    Fingerprint

    Microscopes
    fluorescence
    Fluorescence
    Molecules
    Water
    air
    Air
    wavelength
    Wavelength
    water
    Xenon
    Laser excitation
    xenon
    Wave transmission
    Pyrene
    Electric lamps
    pyrene
    Lighting
    laser

    All Science Journal Classification (ASJC) codes

    • Electronic, Optical and Magnetic Materials
    • Ceramics and Composites
    • Surfaces, Coatings and Films
    • Management, Monitoring, Policy and Law

    Cite this

    @article{3b3c070b066a4e34ab662252fee19dc2,
    title = "Design of a semi-confocal fluorescence microscope for observing excitation spectrum of soluble molecules adsorbed at the air/water interface",
    abstract = "A semi-confocal fluorescence microscope is designed and constructed aiming to observe fluorescence excitation spectrum of soluble molecules adsorbed at the air/water interface. With a conventional confocal fluorescence microscope, the target molecules of pyrene arises some difficulties: excitation wavelength in deep ultraviolet forces low transmission performance of microscopic objectives and low wavelength-tunable performance of an excitation laser. A total internal reflection illumination with a light-guide coupled monochromated-xenon lamp is combined with the confocal fluorescence microscope. Theoretical feasibility and performance of the microscope are discussed with preliminary results obtained.",
    author = "Haiya Yang and Akira Harata",
    year = "2015",
    month = "1",
    day = "1",
    doi = "10.5109/1544074",
    language = "English",
    volume = "2",
    pages = "1--4",
    journal = "Evergreen",
    issn = "2189-0420",
    publisher = "Novel Carbon Resource Sciences",
    number = "2",

    }

    TY - JOUR

    T1 - Design of a semi-confocal fluorescence microscope for observing excitation spectrum of soluble molecules adsorbed at the air/water interface

    AU - Yang, Haiya

    AU - Harata, Akira

    PY - 2015/1/1

    Y1 - 2015/1/1

    N2 - A semi-confocal fluorescence microscope is designed and constructed aiming to observe fluorescence excitation spectrum of soluble molecules adsorbed at the air/water interface. With a conventional confocal fluorescence microscope, the target molecules of pyrene arises some difficulties: excitation wavelength in deep ultraviolet forces low transmission performance of microscopic objectives and low wavelength-tunable performance of an excitation laser. A total internal reflection illumination with a light-guide coupled monochromated-xenon lamp is combined with the confocal fluorescence microscope. Theoretical feasibility and performance of the microscope are discussed with preliminary results obtained.

    AB - A semi-confocal fluorescence microscope is designed and constructed aiming to observe fluorescence excitation spectrum of soluble molecules adsorbed at the air/water interface. With a conventional confocal fluorescence microscope, the target molecules of pyrene arises some difficulties: excitation wavelength in deep ultraviolet forces low transmission performance of microscopic objectives and low wavelength-tunable performance of an excitation laser. A total internal reflection illumination with a light-guide coupled monochromated-xenon lamp is combined with the confocal fluorescence microscope. Theoretical feasibility and performance of the microscope are discussed with preliminary results obtained.

    UR - http://www.scopus.com/inward/record.url?scp=85019578218&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=85019578218&partnerID=8YFLogxK

    U2 - 10.5109/1544074

    DO - 10.5109/1544074

    M3 - Article

    AN - SCOPUS:85019578218

    VL - 2

    SP - 1

    EP - 4

    JO - Evergreen

    JF - Evergreen

    SN - 2189-0420

    IS - 2

    ER -