Design of a semi-confocal fluorescence microscope for observing excitation spectrum of soluble molecules adsorbed at the air/water interface

Haiya Yang, Akira Harata

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    A semi-confocal fluorescence microscope is designed and constructed aiming to observe fluorescence excitation spectrum of soluble molecules adsorbed at the air/water interface. With a conventional confocal fluorescence microscope, the target molecules of pyrene arises some difficulties: excitation wavelength in deep ultraviolet forces low transmission performance of microscopic objectives and low wavelength-tunable performance of an excitation laser. A total internal reflection illumination with a light-guide coupled monochromated-xenon lamp is combined with the confocal fluorescence microscope. Theoretical feasibility and performance of the microscope are discussed with preliminary results obtained.

    Original languageEnglish
    Pages (from-to)1-4
    Number of pages4
    JournalEvergreen
    Volume2
    Issue number2
    DOIs
    Publication statusPublished - 2015

    All Science Journal Classification (ASJC) codes

    • Electronic, Optical and Magnetic Materials
    • Ceramics and Composites
    • Surfaces, Coatings and Films
    • Management, Monitoring, Policy and Law

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