Design of polymer brushes for immobilizing enzymes onto hollow fiber micropores in organic media reaction

Muneharu Goto, Tokie Okubo, Hidetaka Kawakita, Kazuya Uezu, Satoshi Tsuneda, Kyoichi Saito, Masahiro Goto, Masao Tamada, Takanobu Sugo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

To immobilize lipase for enzymatic reactions in organic solvent, various functional [epoxy (GMA-fiber), hydroxyl (OH-fiber) or diethyl amino (DEA-fiber)] groups were introduced onto porous hollow-fiber membranes by radiation-induced graft polymerization of glycidyl methacrylate and chemical modification. Lipase from Candida rugosa was immobilized on polymer brushes by permeation of lipase. The activities of immobilized lipase were measured by esterification reactions between lauric acid and benzyl alcohol in isooctane. The activity of immobilized lipase on GMA-fibers, DEA-fibers and OH-fibers was 0.70 mol/(h kg-lipase), 0.50 mol/(h kg-lipase), and 2.45 mol/(h kg-lipase), respectively. Immobilized lipase on DEA-fibers or OH-fibers was reused three times after it was used in the batch reactor for 24 h. It was found that lipase activity showed no signs of denaturation. However, when native lipase was used, lipase activity remarkably decreased after reusing.

Original languageEnglish
Pages (from-to)159-165
Number of pages7
JournalBiochemical Engineering Journal
Volume37
Issue number2
DOIs
Publication statusPublished - Nov 15 2007

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Environmental Engineering
  • Biomedical Engineering

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