Design of RT-PCR primers method considered by gene family

Ken Ichi Makino; Kenta Tomonaga; Hideyasu Hirano; Masahiro Nakano; Tadahiro Kin; Hlroyuki Matsuura; Kazuharu Koide; Nobuo Noda

Design of RT-PCR primers method considered by gene family

Ken Ichi Makino, Kenta Tomonaga, Hideyasu Hirano, Masahiro Nakano, Tadahiro Kin, Hlroyuki Matsuura, Kazuharu Koide, Nobuo Noda

Engineering Science for Advanced energy system

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10 Citations (Scopus)

Abstract

TO detect mRNA expression levels were very useful not only for the biological studies but also for the progress of medical therapy. Furthermore, mRNA transcription suppression caused by antisense also clearized and made possible the speedy result of the cell biological investigation. We created primer design protocol using fuzzy deducing theory, because most of the traditional computer protocol succeeded less than 20%. 90% of Our primers functioned after following checking (1. GC Contents Check; 8. Self Ho-mology Check; 3. Fuzzy Deducing Homologous Check: [versus a.: GAPDH, b: Profüin, c: β-actin]). We also added 4: gene family checking to target into a real mRNA out of similar sequences, and 5; 5'-, & -3' homology checking. Fuzzy deducing checking protocol made it possible to select real VEGF mRNA from a gene family in which there are many similar seauences. ICIC International

Original language	English
Pages (from-to)	269-274
Number of pages	6
Journal	International Journal of Innovative Computing, Information and Control
Volume	4
Issue number	2
Publication status	Published - Feb 1 2008

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All Science Journal Classification (ASJC) codes

Software
Theoretical Computer Science
Information Systems
Computational Theory and Mathematics

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Makino, K. I., Tomonaga, K., Hirano, H., Nakano, M., Kin, T., Matsuura, H., ... Noda, N. (2008). Design of RT-PCR primers method considered by gene family. International Journal of Innovative Computing, Information and Control, 4(2), 269-274.

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AU - Makino, Ken Ichi

AU - Tomonaga, Kenta

AU - Hirano, Hideyasu

AU - Nakano, Masahiro

AU - Kin, Tadahiro

AU - Matsuura, Hlroyuki

AU - Koide, Kazuharu

AU - Noda, Nobuo

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AB - TO detect mRNA expression levels were very useful not only for the biological studies but also for the progress of medical therapy. Furthermore, mRNA transcription suppression caused by antisense also clearized and made possible the speedy result of the cell biological investigation. We created primer design protocol using fuzzy deducing theory, because most of the traditional computer protocol succeeded less than 20%. 90% of Our primers functioned after following checking (1. GC Contents Check; 8. Self Ho-mology Check; 3. Fuzzy Deducing Homologous Check: [versus a.: GAPDH, b: Profüin, c: β-actin]). We also added 4: gene family checking to target into a real mRNA out of similar sequences, and 5; 5'-, & -3' homology checking. Fuzzy deducing checking protocol made it possible to select real VEGF mRNA from a gene family in which there are many similar seauences. ICIC International

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Design of RT-PCR primers method considered by gene family

Abstract

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All Science Journal Classification (ASJC) codes

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