Design of RT-PCR primers method considered by gene family

Ken Ichi Makino, Kenta Tomonaga, Hideyasu Hirano, Masahiro Nakano, Tadahiro Kin, Hlroyuki Matsuura, Kazuharu Koide, Nobuo Noda

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

TO detect mRNA expression levels were very useful not only for the biological studies but also for the progress of medical therapy. Furthermore, mRNA transcription suppression caused by antisense also clearized and made possible the speedy result of the cell biological investigation. We created primer design protocol using fuzzy deducing theory, because most of the traditional computer protocol succeeded less than 20%. 90% of Our primers functioned after following checking (1. GC Contents Check; 8. Self Ho-mology Check; 3. Fuzzy Deducing Homologous Check: [versus a.: GAPDH, b: Profüin, c: β-actin]). We also added 4: gene family checking to target into a real mRNA out of similar sequences, and 5; 5'-, & -3' homology checking. Fuzzy deducing checking protocol made it possible to select real VEGF mRNA from a gene family in which there are many similar seauences. ICIC International

Original languageEnglish
Pages (from-to)269-274
Number of pages6
JournalInternational Journal of Innovative Computing, Information and Control
Volume4
Issue number2
Publication statusPublished - Feb 1 2008

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All Science Journal Classification (ASJC) codes

  • Software
  • Theoretical Computer Science
  • Information Systems
  • Computational Theory and Mathematics

Cite this

Makino, K. I., Tomonaga, K., Hirano, H., Nakano, M., Kin, T., Matsuura, H., ... Noda, N. (2008). Design of RT-PCR primers method considered by gene family. International Journal of Innovative Computing, Information and Control, 4(2), 269-274.