Design of RT-PCR primers method considered by gene family

Ken Ichi Makino; Kenta Tomonaga; Hideyasu Hirano; Masahiro Nakano; Tadahiro Kin; Hlroyuki Matsuura; Kazuharu Koide; Nobuo Noda

Design of RT-PCR primers method considered by gene family

Ken Ichi Makino, Kenta Tomonaga, Hideyasu Hirano, Masahiro Nakano, Tadahiro Kin, Hlroyuki Matsuura, Kazuharu Koide, Nobuo Noda

Engineering Science for Advanced energy system

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

TO detect mRNA expression levels were very useful not only for the biological studies but also for the progress of medical therapy. Furthermore, mRNA transcription suppression caused by antisense also clearized and made possible the speedy result of the cell biological investigation. We created primer design protocol using fuzzy deducing theory, because most of the traditional computer protocol succeeded less than 20%. 90% of Our primers functioned after following checking (1. GC Contents Check; 8. Self Ho-mology Check; 3. Fuzzy Deducing Homologous Check: [versus a.: GAPDH, b: Profüin, c: β-actin]). We also added 4: gene family checking to target into a real mRNA out of similar sequences, and 5; 5'-, & -3' homology checking. Fuzzy deducing checking protocol made it possible to select real VEGF mRNA from a gene family in which there are many similar seauences. ICIC International

Original language	English
Pages (from-to)	269-274
Number of pages	6
Journal	International Journal of Innovative Computing, Information and Control
Volume	4
Issue number	2
Publication status	Published - Feb 1 2008

All Science Journal Classification (ASJC) codes

Software
Theoretical Computer Science
Information Systems
Computational Theory and Mathematics

Access to Document

Fingerprint Dive into the research topics of 'Design of RT-PCR primers method considered by gene family'. Together they form a unique fingerprint.

Cite this

Design of RT-PCR primers method considered by gene family. / Makino, Ken Ichi; Tomonaga, Kenta; Hirano, Hideyasu; Nakano, Masahiro; Kin, Tadahiro; Matsuura, Hlroyuki; Koide, Kazuharu; Noda, Nobuo.

In: International Journal of Innovative Computing, Information and Control, Vol. 4, No. 2, 01.02.2008, p. 269-274.

Research output: Contribution to journal › Article › peer-review

@article{7ca227cb78b44cea8c3f18e617366960,

title = "Design of RT-PCR primers method considered by gene family",

abstract = "TO detect mRNA expression levels were very useful not only for the biological studies but also for the progress of medical therapy. Furthermore, mRNA transcription suppression caused by antisense also clearized and made possible the speedy result of the cell biological investigation. We created primer design protocol using fuzzy deducing theory, because most of the traditional computer protocol succeeded less than 20%. 90% of Our primers functioned after following checking (1. GC Contents Check; 8. Self Ho-mology Check; 3. Fuzzy Deducing Homologous Check: [versus a.: GAPDH, b: Prof{\"u}in, c: β-actin]). We also added 4: gene family checking to target into a real mRNA out of similar sequences, and 5; 5'-, & -3' homology checking. Fuzzy deducing checking protocol made it possible to select real VEGF mRNA from a gene family in which there are many similar seauences. ICIC International",

author = "Makino, {Ken Ichi} and Kenta Tomonaga and Hideyasu Hirano and Masahiro Nakano and Tadahiro Kin and Hlroyuki Matsuura and Kazuharu Koide and Nobuo Noda",

year = "2008",

month = feb,

day = "1",

language = "English",

volume = "4",

pages = "269--274",

journal = "International Journal of Innovative Computing, Information and Control",

issn = "1349-4198",

publisher = "IJICIC Editorial Office",

number = "2",

}

TY - JOUR

T1 - Design of RT-PCR primers method considered by gene family

AU - Makino, Ken Ichi

AU - Tomonaga, Kenta

AU - Hirano, Hideyasu

AU - Nakano, Masahiro

AU - Kin, Tadahiro

AU - Matsuura, Hlroyuki

AU - Koide, Kazuharu

AU - Noda, Nobuo

PY - 2008/2/1

Y1 - 2008/2/1

N2 - TO detect mRNA expression levels were very useful not only for the biological studies but also for the progress of medical therapy. Furthermore, mRNA transcription suppression caused by antisense also clearized and made possible the speedy result of the cell biological investigation. We created primer design protocol using fuzzy deducing theory, because most of the traditional computer protocol succeeded less than 20%. 90% of Our primers functioned after following checking (1. GC Contents Check; 8. Self Ho-mology Check; 3. Fuzzy Deducing Homologous Check: [versus a.: GAPDH, b: Profüin, c: β-actin]). We also added 4: gene family checking to target into a real mRNA out of similar sequences, and 5; 5'-, & -3' homology checking. Fuzzy deducing checking protocol made it possible to select real VEGF mRNA from a gene family in which there are many similar seauences. ICIC International

AB - TO detect mRNA expression levels were very useful not only for the biological studies but also for the progress of medical therapy. Furthermore, mRNA transcription suppression caused by antisense also clearized and made possible the speedy result of the cell biological investigation. We created primer design protocol using fuzzy deducing theory, because most of the traditional computer protocol succeeded less than 20%. 90% of Our primers functioned after following checking (1. GC Contents Check; 8. Self Ho-mology Check; 3. Fuzzy Deducing Homologous Check: [versus a.: GAPDH, b: Profüin, c: β-actin]). We also added 4: gene family checking to target into a real mRNA out of similar sequences, and 5; 5'-, & -3' homology checking. Fuzzy deducing checking protocol made it possible to select real VEGF mRNA from a gene family in which there are many similar seauences. ICIC International

UR - http://www.scopus.com/inward/record.url?scp=63649121161&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=63649121161&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:63649121161

VL - 4

SP - 269

EP - 274

JO - International Journal of Innovative Computing, Information and Control

JF - International Journal of Innovative Computing, Information and Control

SN - 1349-4198

IS - 2

ER -