TY - JOUR
T1 - Design, synthesis and characterization of a novel fluorescent probe for nitric oxide (nitrogen monoxide)
AU - Katayama, Yoshiki
AU - Takahashi, Shinji
AU - Maeda, Mizuo
N1 - Funding Information:
This work was supported in part by The Asahi Glass Foundation of Japan. Financial support by a Grant-in-Aid (09750887) for Scientific Research from the Ministry of Education, Science and Culture of Japan is also acknowledged.
Copyright:
Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.
PY - 1998/6/5
Y1 - 1998/6/5
N2 - The iron(II) complex of quinoline pendant cyclam was designed as a mimic of the activation site of guanylate cyclase and synthesized as a fluorescent probe for nitric oxide (NO). Fe(II) ion shifted the excitation and emission maxima of the pendant fluorophore to longer wavelengths, and the fluorescence intensity was greatly increased. NO from NO-releasing agents quenched the fluorescence effectively. Nitrite, which is a metabolite of NO, did not affect the fluorescence of the complex. Cyanate also did not affect the spectral change of the complex with NO. NO, as low as μM, can be determined spectrofluorimetrically using 200μM reagent. This is the first example of a fluorescent probe for NO which recognizes the molecule directly. Copyright (C) 1998 Elsevier Science B.V.
AB - The iron(II) complex of quinoline pendant cyclam was designed as a mimic of the activation site of guanylate cyclase and synthesized as a fluorescent probe for nitric oxide (NO). Fe(II) ion shifted the excitation and emission maxima of the pendant fluorophore to longer wavelengths, and the fluorescence intensity was greatly increased. NO from NO-releasing agents quenched the fluorescence effectively. Nitrite, which is a metabolite of NO, did not affect the fluorescence of the complex. Cyanate also did not affect the spectral change of the complex with NO. NO, as low as μM, can be determined spectrofluorimetrically using 200μM reagent. This is the first example of a fluorescent probe for NO which recognizes the molecule directly. Copyright (C) 1998 Elsevier Science B.V.
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U2 - 10.1016/S0003-2670(98)00089-0
DO - 10.1016/S0003-2670(98)00089-0
M3 - Article
AN - SCOPUS:0031781070
VL - 365
SP - 159
EP - 167
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 1-3
ER -