Design, synthesis and photophysical properties of C60-modified proteins

Hiroto Murakami, Rika Matsumoto, Yuko Okusa, Takamasa Sagara, Mamoru Fujitsuka, Osamu Ito, Naotoshi Nakashima

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16 Citations (Scopus)

Abstract

Fullerene-porphyrin conjugates coordinated with Fe and Zn ions (1·Fe and 1·Zn) were designed and synthesized. Compounds 1·Fe and 1·Zn were reconstituted into apomyoglobin successfully to produce C60-modified myoglobins, 1·Fe-Mb and 1·Zn-Mb, respectively. The axial-ligand exchange reaction revealed that 1·Fe-Mb maintains the intrinsic properties of native Mb, except for the autooxidation rate constant, suggesting similar microenvironment of the porphyrin moieties in the proteins. Cyclic voltammogram (CV) of a graphite electrode modified with a film of 1·Fe-Mb-didodecyldimethylammonium bromide (DDAB) showed two reversible redox couples with E0 = -206 and -1048 mV which are attributable to Fe2+/3+ and the reduction of the porphyrin ring, respectively. Differential pulse voltammogram of an electrode modified with a 1·Fe-Mb-tridodecylmethylammonium bromide (TDAB) film in water containing 0.5 M tetraethylammonium chloride and 10 mM 2,2′,2″-nitrilotriethanol showed three cathodic peaks at E1/2.1 = -372, E1/2.2 = -555, and E1/2.3 = -1028 mV which are attributable to Fe2+/3+, C600/1-, and the reduction of the porphyrin ring, respectively. The electrodes modified with 1·Fe-Mb and 1·Zn-Mb gave anodic photocurrent coupled with on-off light irradiation. The action spectrum of photocurrent for a 1·Zn-Mb-DDAB film was in accord with the UV-vis absorption spectrum of 1·Zn-Mb. Transient absorption spectra of 1·Zn in benzonitrile and 1·Zn-Mb in a 50 mM phosphate buffer at 100 ns after the ns-laser light pulse irradiation at 532 nm showed three absorption maxima at 700, 830, and 1000 nm which are assignable to the triplet excited state of C60 (3C60*), the triplet excited state of the zinc porphyrin (3Znp*), and C60 radical anion (C60.-), respectively. Existence of the apparent C60.- indicates the generation of charge-separation state, ZnP.+-C60.-. The rate constants for the generation of the charge separation states in 1·Zn and 1·Zn-Mb calculated from the fluorescence data were 8.2 × 108 s-1 and 6.4 × 108 s-1, respectively, and the corresponding quantum yields were 0.62 and 0.59.

Original languageEnglish
Pages (from-to)2026-2033
Number of pages8
JournalJournal of Materials Chemistry
Volume12
Issue number7
DOIs
Publication statusPublished - Jan 1 2002

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Chemistry

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