Detection and inhibition of lipid-derived radicals in low-density lipoprotein

Yuma Ishida, Yuka Okamoto, Yuta Matsuoka, Arisa Tada, Jindaporn Janprasit, Mayumi Yamato, Noppawan Phumala Morales, Ken Ichi Yamada

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Oxidized low density lipoprotein (Ox-LDL) is implicated in a variety of oxidative diseases. To clarify the mechanisms involved and facilitate the investigation of therapeutics, we previously developed a detection method for lipid-derived radicals using the fluorescent probe 2,2,6-trimethyl-6-pentyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)piperidine-1-oxyl (NBD-Pen). In this study, NBD-Pen was used to detect lipid-derived radicals in Ox-LDL from in vitro and in vivo samples using an iron overloaded mouse model. By following the timeline of lipid radical generation using this method, the iron overloaded mice could be successfully treated with the antioxidant Trolox, resulting in successful lowering of the plasma lipid peroxidation, aspartate transaminase and alanine transaminase levels. Furthermore, using a combination therapy of the chelating agent deferoxamine (DFX) and Trolox, liver injury and oxidative stress markers were also reduced in iron overloaded mice. The NBD-Pen method is highly sensitive as well as selective and is suitable for targeting minimally modified LDL compared with other existing methods.

Original languageEnglish
Pages (from-to)487-493
Number of pages7
JournalFree Radical Biology and Medicine
Volume113
DOIs
Publication statusPublished - Dec 2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology (medical)

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