Abstract
Oxidized low density lipoprotein (Ox-LDL) is implicated in a variety of oxidative diseases. To clarify the mechanisms involved and facilitate the investigation of therapeutics, we previously developed a detection method for lipid-derived radicals using the fluorescent probe 2,2,6-trimethyl-6-pentyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)piperidine-1-oxyl (NBD-Pen). In this study, NBD-Pen was used to detect lipid-derived radicals in Ox-LDL from in vitro and in vivo samples using an iron overloaded mouse model. By following the timeline of lipid radical generation using this method, the iron overloaded mice could be successfully treated with the antioxidant Trolox, resulting in successful lowering of the plasma lipid peroxidation, aspartate transaminase and alanine transaminase levels. Furthermore, using a combination therapy of the chelating agent deferoxamine (DFX) and Trolox, liver injury and oxidative stress markers were also reduced in iron overloaded mice. The NBD-Pen method is highly sensitive as well as selective and is suitable for targeting minimally modified LDL compared with other existing methods.
| Original language | English |
|---|---|
| Pages (from-to) | 487-493 |
| Number of pages | 7 |
| Journal | Free Radical Biology and Medicine |
| Volume | 113 |
| DOIs | |
| Publication status | Published - Dec 1 2017 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Physiology (medical)
Cite this
Detection and inhibition of lipid-derived radicals in low-density lipoprotein. / Ishida, Yuma; Okamoto, Yuka; Matsuoka, Yuta; Tada, Arisa; Janprasit, Jindaporn; Yamato, Mayumi; Morales, Noppawan Phumala; Yamada, Ken-Ichi.
In: Free Radical Biology and Medicine, Vol. 113, 01.12.2017, p. 487-493.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection and inhibition of lipid-derived radicals in low-density lipoprotein
AU - Ishida, Yuma
AU - Okamoto, Yuka
AU - Matsuoka, Yuta
AU - Tada, Arisa
AU - Janprasit, Jindaporn
AU - Yamato, Mayumi
AU - Morales, Noppawan Phumala
AU - Yamada, Ken-Ichi
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Oxidized low density lipoprotein (Ox-LDL) is implicated in a variety of oxidative diseases. To clarify the mechanisms involved and facilitate the investigation of therapeutics, we previously developed a detection method for lipid-derived radicals using the fluorescent probe 2,2,6-trimethyl-6-pentyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)piperidine-1-oxyl (NBD-Pen). In this study, NBD-Pen was used to detect lipid-derived radicals in Ox-LDL from in vitro and in vivo samples using an iron overloaded mouse model. By following the timeline of lipid radical generation using this method, the iron overloaded mice could be successfully treated with the antioxidant Trolox, resulting in successful lowering of the plasma lipid peroxidation, aspartate transaminase and alanine transaminase levels. Furthermore, using a combination therapy of the chelating agent deferoxamine (DFX) and Trolox, liver injury and oxidative stress markers were also reduced in iron overloaded mice. The NBD-Pen method is highly sensitive as well as selective and is suitable for targeting minimally modified LDL compared with other existing methods.
AB - Oxidized low density lipoprotein (Ox-LDL) is implicated in a variety of oxidative diseases. To clarify the mechanisms involved and facilitate the investigation of therapeutics, we previously developed a detection method for lipid-derived radicals using the fluorescent probe 2,2,6-trimethyl-6-pentyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)piperidine-1-oxyl (NBD-Pen). In this study, NBD-Pen was used to detect lipid-derived radicals in Ox-LDL from in vitro and in vivo samples using an iron overloaded mouse model. By following the timeline of lipid radical generation using this method, the iron overloaded mice could be successfully treated with the antioxidant Trolox, resulting in successful lowering of the plasma lipid peroxidation, aspartate transaminase and alanine transaminase levels. Furthermore, using a combination therapy of the chelating agent deferoxamine (DFX) and Trolox, liver injury and oxidative stress markers were also reduced in iron overloaded mice. The NBD-Pen method is highly sensitive as well as selective and is suitable for targeting minimally modified LDL compared with other existing methods.
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UR - http://www.scopus.com/inward/citedby.url?scp=85032684862&partnerID=8YFLogxK
U2 - 10.1016/j.freeradbiomed.2017.10.388
DO - 10.1016/j.freeradbiomed.2017.10.388
M3 - Article
C2 - 29107744
AN - SCOPUS:85032684862
VL - 113
SP - 487
EP - 493
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
SN - 0891-5849
ER -