Detection and quantification of ginsenoside Re in ginseng samples by a chromatographic immunostaining method using monoclonal antibody against ginsenoside Re

Osamu Morinaga; Hiroyuki Tanaka; Yukihiro Shoyama

doi:10.1016/j.jchromb.2005.10.040

Detection and quantification of ginsenoside Re in ginseng samples by a chromatographic immunostaining method using monoclonal antibody against ginsenoside Re

Osamu Morinaga, Hiroyuki Tanaka, Yukihiro Shoyama

Pharmaceutical Health Care and Sciences

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42 Citations (Scopus)

Abstract

A chromatographic immunostaining method has been developed for the determination of ginsenoside Re (G-Re) in ginseng samples on a polyethersulphone (PES) membrane. G-Re standard and the extracts of ginseng roots were applied to a PES membrane and developed by methanol-water-acetic acid (45:55:1, by volume). G-Re was clearly detected by an immunostaining method using a monoclonal antibody against G-Re. The coloring spots of G-Re were analyzed quantitatively using NIH Image software indicating at least 0.125 μg of G-Re was detectable. G-Re can be analyzed quantitatively between 0.25 and 4.0 μg.

Original language	English
Pages (from-to)	100-104
Number of pages	5
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	830
Issue number	1
DOIs	https://doi.org/10.1016/j.jchromb.2005.10.040
Publication status	Published - Jan 2 2006

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

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10.1016/j.jchromb.2005.10.040

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Detection and quantification of ginsenoside Re in ginseng samples by a chromatographic immunostaining method using monoclonal antibody against ginsenoside Re. / Morinaga, Osamu; Tanaka, Hiroyuki; Shoyama, Yukihiro.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 830, No. 1, 02.01.2006, p. 100-104.

Research output: Contribution to journal › Article › peer-review

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Detection and quantification of ginsenoside Re in ginseng samples by a chromatographic immunostaining method using monoclonal antibody against ginsenoside Re

Abstract

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