Detection of adenovirus E1A DNA in pulmonary fibrosis using nested polymerase chain reaction

K. Kuwano, Y. Nomoto, R. Kunitake, N. Hagimoto, T. Matsuba, Yoichi Nakanishi, N. Hara

Research output: Contribution to journalArticle

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Abstract

The history of patients with idiopathic pulmonary fibrosis (IPF) shows that the disease my be preceded by a vital-like illness. Although viruses have not been demonstrated, it is possible that viruses were not detected in culture because they do not replicate during latency. We investigated the presence of adenovirus in IPF and interstitial pneumonia associated with collagen vascular disease (CVD-IP), using the nested polymerase chain reaction (PCR) and in situ hybridization (ISE) for the E1A region of the adenovirus genome. Studies were performed on lung tissues obtained by transbronchial lung biopsy from 19 patients with IPF, 10 patients with CVD- IP and, for comparison, from 20 patients with sarcoidosis. The E1A DNA was present in 3 out of 19 (16%) cases of IPF, in 5 of 10 (50%) cases of CVD-IP, and in 2 of 20 (10%) cases of sarcoidosis. The incidence of E1A DNA in CVD- IP was significantly higher than that in sarcoidosis (p<0.05). In patients with IPF and CVD-IP, E1A DNA was more prevalent in patients treated with corticosteroids (6 out of 9 cases; 67%) than in those without it (2 out of 20 cases; 10%) (p<0.01). ISH studies showed that I out of 8 cases of IPF and CVD-IP, in which E1A DNA was detected by PCR, was positive for E1A DNA. We conclude that adenovirus E1A is unlikely to be aetiologically involved in the pathogenesis of idiopathic pulmonary fibrosis or interstitial pneumonia associated with collagen vascular disease. However, a latent adenovirus infection may be reactivated or may newly infect the hast following corticosteroid administration.

Original languageEnglish
Pages (from-to)1445-1449
Number of pages5
JournalEuropean Respiratory Journal
Volume10
Issue number7
DOIs
Publication statusPublished - Jul 1 1997

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Idiopathic Pulmonary Fibrosis
Pulmonary Fibrosis
Adenoviridae
Polymerase Chain Reaction
DNA
Sarcoidosis
Collagen Diseases
Interstitial Lung Diseases
Vascular Diseases
Adrenal Cortex Hormones
Viruses
Adenoviridae Infections
Lung
In Situ Hybridization
History
Genome
Biopsy
Incidence

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cite this

Kuwano, K., Nomoto, Y., Kunitake, R., Hagimoto, N., Matsuba, T., Nakanishi, Y., & Hara, N. (1997). Detection of adenovirus E1A DNA in pulmonary fibrosis using nested polymerase chain reaction. European Respiratory Journal, 10(7), 1445-1449. https://doi.org/10.1183/09031936.97.10071445

Detection of adenovirus E1A DNA in pulmonary fibrosis using nested polymerase chain reaction. / Kuwano, K.; Nomoto, Y.; Kunitake, R.; Hagimoto, N.; Matsuba, T.; Nakanishi, Yoichi; Hara, N.

In: European Respiratory Journal, Vol. 10, No. 7, 01.07.1997, p. 1445-1449.

Research output: Contribution to journalArticle

Kuwano, K, Nomoto, Y, Kunitake, R, Hagimoto, N, Matsuba, T, Nakanishi, Y & Hara, N 1997, 'Detection of adenovirus E1A DNA in pulmonary fibrosis using nested polymerase chain reaction', European Respiratory Journal, vol. 10, no. 7, pp. 1445-1449. https://doi.org/10.1183/09031936.97.10071445
Kuwano, K. ; Nomoto, Y. ; Kunitake, R. ; Hagimoto, N. ; Matsuba, T. ; Nakanishi, Yoichi ; Hara, N. / Detection of adenovirus E1A DNA in pulmonary fibrosis using nested polymerase chain reaction. In: European Respiratory Journal. 1997 ; Vol. 10, No. 7. pp. 1445-1449.
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