Detection of base pairing of an oxidatively damaged guanine using colloidal stability change of DNA-linked polymer micelles.

Tohru Takarada, Emi Imaizumi, Katsuhito Kino, Keitaro Yoshimoto, Shinzi Ogasawara, Naoki Kanayama, Mizuo Maeda

Research output: Contribution to journalArticle

Abstract

A DNA-linked polymer micelle was prepared through a phase transition of an amphiphilic copolymer consisting of single-stranded DNA as the hydrophilic part and thermo-responsive poly(N-isopropylacryl-amide) as the hydrophobic part. Duplex formation on the surface of micelle decreased the colloidal stability, whereas a terminal single-base mismatch at the interface between the DNA shell and the disperse medium increased the colloidal stability. Based on this change, we successfully detected base pairing of 7,8-dihydro-8-oxo-2'-deoxyguanine with adenine. This method will be useful for discovering a Watson-Crick or non Watson-Crick base pair of an unknown oxidative-damaged base with each four canonical one.

Original languageEnglish
Pages (from-to)305-306
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

Guanine
Micelles
Base Pairing
Polymers
Single-Stranded DNA
DNA
Phase Transition
Adenine
Amides

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Detection of base pairing of an oxidatively damaged guanine using colloidal stability change of DNA-linked polymer micelles. / Takarada, Tohru; Imaizumi, Emi; Kino, Katsuhito; Yoshimoto, Keitaro; Ogasawara, Shinzi; Kanayama, Naoki; Maeda, Mizuo.

In: Nucleic acids symposium series (2004), No. 51, 2007, p. 305-306.

Research output: Contribution to journalArticle

Takarada, Tohru ; Imaizumi, Emi ; Kino, Katsuhito ; Yoshimoto, Keitaro ; Ogasawara, Shinzi ; Kanayama, Naoki ; Maeda, Mizuo. / Detection of base pairing of an oxidatively damaged guanine using colloidal stability change of DNA-linked polymer micelles. In: Nucleic acids symposium series (2004). 2007 ; No. 51. pp. 305-306.
@article{8349a4362174481fab45b5ac69c5ab16,
title = "Detection of base pairing of an oxidatively damaged guanine using colloidal stability change of DNA-linked polymer micelles.",
abstract = "A DNA-linked polymer micelle was prepared through a phase transition of an amphiphilic copolymer consisting of single-stranded DNA as the hydrophilic part and thermo-responsive poly(N-isopropylacryl-amide) as the hydrophobic part. Duplex formation on the surface of micelle decreased the colloidal stability, whereas a terminal single-base mismatch at the interface between the DNA shell and the disperse medium increased the colloidal stability. Based on this change, we successfully detected base pairing of 7,8-dihydro-8-oxo-2'-deoxyguanine with adenine. This method will be useful for discovering a Watson-Crick or non Watson-Crick base pair of an unknown oxidative-damaged base with each four canonical one.",
author = "Tohru Takarada and Emi Imaizumi and Katsuhito Kino and Keitaro Yoshimoto and Shinzi Ogasawara and Naoki Kanayama and Mizuo Maeda",
year = "2007",
language = "English",
pages = "305--306",
journal = "Nucleic acids symposium series (2004)",
issn = "1746-8272",
publisher = "Oxford University Press",
number = "51",

}

TY - JOUR

T1 - Detection of base pairing of an oxidatively damaged guanine using colloidal stability change of DNA-linked polymer micelles.

AU - Takarada, Tohru

AU - Imaizumi, Emi

AU - Kino, Katsuhito

AU - Yoshimoto, Keitaro

AU - Ogasawara, Shinzi

AU - Kanayama, Naoki

AU - Maeda, Mizuo

PY - 2007

Y1 - 2007

N2 - A DNA-linked polymer micelle was prepared through a phase transition of an amphiphilic copolymer consisting of single-stranded DNA as the hydrophilic part and thermo-responsive poly(N-isopropylacryl-amide) as the hydrophobic part. Duplex formation on the surface of micelle decreased the colloidal stability, whereas a terminal single-base mismatch at the interface between the DNA shell and the disperse medium increased the colloidal stability. Based on this change, we successfully detected base pairing of 7,8-dihydro-8-oxo-2'-deoxyguanine with adenine. This method will be useful for discovering a Watson-Crick or non Watson-Crick base pair of an unknown oxidative-damaged base with each four canonical one.

AB - A DNA-linked polymer micelle was prepared through a phase transition of an amphiphilic copolymer consisting of single-stranded DNA as the hydrophilic part and thermo-responsive poly(N-isopropylacryl-amide) as the hydrophobic part. Duplex formation on the surface of micelle decreased the colloidal stability, whereas a terminal single-base mismatch at the interface between the DNA shell and the disperse medium increased the colloidal stability. Based on this change, we successfully detected base pairing of 7,8-dihydro-8-oxo-2'-deoxyguanine with adenine. This method will be useful for discovering a Watson-Crick or non Watson-Crick base pair of an unknown oxidative-damaged base with each four canonical one.

UR - http://www.scopus.com/inward/record.url?scp=42949109292&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42949109292&partnerID=8YFLogxK

M3 - Article

C2 - 18029708

AN - SCOPUS:42949109292

SP - 305

EP - 306

JO - Nucleic acids symposium series (2004)

JF - Nucleic acids symposium series (2004)

SN - 1746-8272

IS - 51

ER -