表面プラズモン共鳴センサを用いた尿中カテコールアミン代謝物の検出

Translated title of the contribution: Detection of catecholamine metabolite in urine using surface plasmon resonance sensor

Takeshi Onodera, Takuya Eda, Hideto Sonoda, Toshiro Matsui, Kiyoshi Toko

Research output: Contribution to journalArticle

Abstract

In this study, an Au sensor surface of surface plasmon resonance was developed for detection of catecholamine metabolites such as homovanilic acid (HVA) in urine samples. The sensor surface was modified with mixed self-assembled monolayer containing oligo (ethylene glycol) chain for reducing nonspecific adsorption. An indirect competitive assay using anti-HVA-concholepas concholepas hemocyanin (CCH) polyclonal antibody and HVA in model of urine solution was carried out. HVA concentrations of human urine samples were estimated from SPR sensor responses by referring the response curve. As the results, the detection limit of HVA in model of urine was obtained 15 ppb. The rank of estimated concentration in five human urine samples was matched with that of the quantified concentration by high performance liquid chromatography-mass spectrometry.

Original languageJapanese
Pages (from-to)278-282
Number of pages5
JournalIEEJ Transactions on Sensors and Micromachines
Volume139
Issue number9
DOIs
Publication statusPublished - Jan 1 2019

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Surface plasmon resonance
Metabolites
Acids
Sensors
High performance liquid chromatography
Self assembled monolayers
Ethylene glycol
Antibodies
Mass spectrometry
Assays
Catecholamines
Adsorption

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

表面プラズモン共鳴センサを用いた尿中カテコールアミン代謝物の検出. / Onodera, Takeshi; Eda, Takuya; Sonoda, Hideto; Matsui, Toshiro; Toko, Kiyoshi.

In: IEEJ Transactions on Sensors and Micromachines, Vol. 139, No. 9, 01.01.2019, p. 278-282.

Research output: Contribution to journalArticle

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