TY - JOUR
T1 - Detection of complement receptors on head kidney phagocytes of the common carp Cyprinus carpio
AU - Nakao, Miki
AU - Fujiki, Kazuhiro
AU - Kondo, Masakazu
AU - Yano, Tomoki
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2003/10
Y1 - 2003/10
N2 - Opsonization is a crucial function of the complement system in immune clearance. To identify receptors responsible for the complement-mediated opsonization in teleost, head kidney leukocytes from β-1,3-glucan-injected and non-injected common carp (Cyprinus carpio) were subjected to a rosette-forming assay with fixed sheep erythrocytes (Ef). Leukocyte fractions rich in granulocytes and macrophages from the glucan-injected carp had an ability to form the rosette with complement-coated Ef ut the corresponding fractions from non-injected fish did not, suggesting that β-1,3-glucan elicits expression of complement receptors on those cells. While sensitization of Ef with antibodies slightly elevated the rosette formation, complement-coated Ef showed a significant increase in rosette formation and this formation was inhibited with anti-carp C3. Rosette formation was also inhibited by EDTA but not by EGTA. These results suggest that complement-dependent opsonization in carp is mediated by CS-receptors analogous to mammalian complement receptor type 3 (CD11b/CD18 or integrin αM/β2), which recognizes the iC3b fragment in a divalent cation-dependent manner.
AB - Opsonization is a crucial function of the complement system in immune clearance. To identify receptors responsible for the complement-mediated opsonization in teleost, head kidney leukocytes from β-1,3-glucan-injected and non-injected common carp (Cyprinus carpio) were subjected to a rosette-forming assay with fixed sheep erythrocytes (Ef). Leukocyte fractions rich in granulocytes and macrophages from the glucan-injected carp had an ability to form the rosette with complement-coated Ef ut the corresponding fractions from non-injected fish did not, suggesting that β-1,3-glucan elicits expression of complement receptors on those cells. While sensitization of Ef with antibodies slightly elevated the rosette formation, complement-coated Ef showed a significant increase in rosette formation and this formation was inhibited with anti-carp C3. Rosette formation was also inhibited by EDTA but not by EGTA. These results suggest that complement-dependent opsonization in carp is mediated by CS-receptors analogous to mammalian complement receptor type 3 (CD11b/CD18 or integrin αM/β2), which recognizes the iC3b fragment in a divalent cation-dependent manner.
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U2 - 10.1046/j.1444-2906.2003.00709.x
DO - 10.1046/j.1444-2906.2003.00709.x
M3 - Article
AN - SCOPUS:0242540930
VL - 69
SP - 929
EP - 935
JO - Fisheries Science
JF - Fisheries Science
SN - 0919-9268
IS - 5
ER -