Detection of cry-type gene homologues in the draft genome sequence of paenibacillus popilliae ATCC 14706t and trial for the expression of recombinant proteins in escherichia coli

Akio Kawahara, Kazuhiro Iiyama, Shin Ichiro Asano, Oumi Nishi, Chisa Yasunaga-Aoki

Research output: Contribution to journalArticlepeer-review

Abstract

Two novel cry-type gene homologues, tentatively named cryPp1 and cryPp2, were detected in the genomic DNA of Paenibacillus popilliae ATCC 14706T, both of which were similar to genes encoding Cry18 proteins, suggesting their similar modes of action. Ours is the first report concerning multiple Cry homologues, detected from a sole strain of P. popilliae. In order to characterize the roles of the two Cry homologues in pathogenicity, a trial for the expression of recombinant proteins in Escherichia coli was conducted, which revealed that the optimization of rare codons, such as supplementation of tRNA genes, was necessary.

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalJournal of Insect Biotechnology and Sericology
Volume89
Issue number2
DOIs
Publication statusPublished - 2020

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

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