Abstract
Analyses of bacterial genes expressed in response to the host environment provide clues to understanding the host-pathogen interactions that lead to the establishment of infection. In this study, a novel method named In Vivo Expressed-Tag ImmunoPrecipitation (IVET-PI) was developed for detecting genes expressed in bacteria that are recovered in a small numbers from host tissues. IVET-IP was designed to overcome some drawbacks of previous similar methods. We applied IVET-IP to Bordetella bronchiseptica colonizing rat trachea and identified 173 genes that were expressed in the bacteria over the entire course of an infection. These gene products included two transcriptional factors that are involved in the expression of filamentous hemagglutinin, adenylate cyclase toxin, and major virulence factors for the bordetellae. We consider that this method might provide novel insight into the course of Bordetella infection.
| Original language | English |
|---|---|
| Pages (from-to) | 249-261 |
| Number of pages | 13 |
| Journal | MICROBIOLOGY and IMMUNOLOGY |
| Volume | 59 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 1 2015 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Immunology
- Virology
Cite this
Detection of genes expressed in Bordetella bronchiseptica colonizing rat trachea by in vivo expressed-tag immunoprecipitation method. / Abe, Hiroyuki; Kamitani, Shigeki; Fukui-Miyazaki, Aya; Shinzawa, Naoaki; Nakamura, Keiji; Horiguchi, Yasuhiko.
In: MICROBIOLOGY and IMMUNOLOGY, Vol. 59, No. 5, 01.05.2015, p. 249-261.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of genes expressed in Bordetella bronchiseptica colonizing rat trachea by in vivo expressed-tag immunoprecipitation method
AU - Abe, Hiroyuki
AU - Kamitani, Shigeki
AU - Fukui-Miyazaki, Aya
AU - Shinzawa, Naoaki
AU - Nakamura, Keiji
AU - Horiguchi, Yasuhiko
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Analyses of bacterial genes expressed in response to the host environment provide clues to understanding the host-pathogen interactions that lead to the establishment of infection. In this study, a novel method named In Vivo Expressed-Tag ImmunoPrecipitation (IVET-PI) was developed for detecting genes expressed in bacteria that are recovered in a small numbers from host tissues. IVET-IP was designed to overcome some drawbacks of previous similar methods. We applied IVET-IP to Bordetella bronchiseptica colonizing rat trachea and identified 173 genes that were expressed in the bacteria over the entire course of an infection. These gene products included two transcriptional factors that are involved in the expression of filamentous hemagglutinin, adenylate cyclase toxin, and major virulence factors for the bordetellae. We consider that this method might provide novel insight into the course of Bordetella infection.
AB - Analyses of bacterial genes expressed in response to the host environment provide clues to understanding the host-pathogen interactions that lead to the establishment of infection. In this study, a novel method named In Vivo Expressed-Tag ImmunoPrecipitation (IVET-PI) was developed for detecting genes expressed in bacteria that are recovered in a small numbers from host tissues. IVET-IP was designed to overcome some drawbacks of previous similar methods. We applied IVET-IP to Bordetella bronchiseptica colonizing rat trachea and identified 173 genes that were expressed in the bacteria over the entire course of an infection. These gene products included two transcriptional factors that are involved in the expression of filamentous hemagglutinin, adenylate cyclase toxin, and major virulence factors for the bordetellae. We consider that this method might provide novel insight into the course of Bordetella infection.
UR - http://www.scopus.com/inward/record.url?scp=84929755509&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84929755509&partnerID=8YFLogxK
U2 - 10.1111/1348-0421.12247
DO - 10.1111/1348-0421.12247
M3 - Article
C2 - 25683445
AN - SCOPUS:84929755509
VL - 59
SP - 249
EP - 261
JO - Microbiology and Immunology
JF - Microbiology and Immunology
SN - 0385-5600
IS - 5
ER -