Detection of Giant Myofibrillar Proteins Connectin and Nebulin by Electrophoresis in 2% Polyacrylamide Slab Gels Strengthened with Agarose

R. Tatsumi, A. Hattori

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

We have established an improved method of sodium dodecyl sulfate-polyacrylamide gel electrophoresis to facilitate analysis of giant myofibrillar proteins connectin and nebulin, whose molecular masses are about 3000 and 700 kDa, respectively. This method consists of 2% polyacrylamide slab gels strengthened with agarose and a specific buffer system. Our 2% polyacrylamide slab gels are superior to usual 1.8 or 2% polyacrylamide disc gels in preparation, handling, and resolution.

Original languageEnglish
Pages (from-to)28-31
Number of pages4
JournalAnalytical Biochemistry
Volume224
Issue number1
DOIs
Publication statusPublished - Jan 1 1995

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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