Detection of Giant Myofibrillar Proteins Connectin and Nebulin by Electrophoresis in 2% Polyacrylamide Slab Gels Strengthened with Agarose

Ryuichi Tatsumi, A. Hattori

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

We have established an improved method of sodium dodecyl sulfate-polyacrylamide gel electrophoresis to facilitate analysis of giant myofibrillar proteins connectin and nebulin, whose molecular masses are about 3000 and 700 kDa, respectively. This method consists of 2% polyacrylamide slab gels strengthened with agarose and a specific buffer system. Our 2% polyacrylamide slab gels are superior to usual 1.8 or 2% polyacrylamide disc gels in preparation, handling, and resolution.

Original languageEnglish
Pages (from-to)28-31
Number of pages4
JournalAnalytical Biochemistry
Volume224
Issue number1
DOIs
Publication statusPublished - Jan 1 1995
Externally publishedYes

Fingerprint

Connectin
Electrophoresis
Sepharose
Proteins
Molecular mass
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Buffers
Gels
polyacrylamide gels
nebulin

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{32c0c28cb34042ee8c72d9d7647650fb,
title = "Detection of Giant Myofibrillar Proteins Connectin and Nebulin by Electrophoresis in 2{\%} Polyacrylamide Slab Gels Strengthened with Agarose",
abstract = "We have established an improved method of sodium dodecyl sulfate-polyacrylamide gel electrophoresis to facilitate analysis of giant myofibrillar proteins connectin and nebulin, whose molecular masses are about 3000 and 700 kDa, respectively. This method consists of 2{\%} polyacrylamide slab gels strengthened with agarose and a specific buffer system. Our 2{\%} polyacrylamide slab gels are superior to usual 1.8 or 2{\%} polyacrylamide disc gels in preparation, handling, and resolution.",
author = "Ryuichi Tatsumi and A. Hattori",
year = "1995",
month = "1",
day = "1",
doi = "10.1006/abio.1995.1004",
language = "English",
volume = "224",
pages = "28--31",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Detection of Giant Myofibrillar Proteins Connectin and Nebulin by Electrophoresis in 2% Polyacrylamide Slab Gels Strengthened with Agarose

AU - Tatsumi, Ryuichi

AU - Hattori, A.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - We have established an improved method of sodium dodecyl sulfate-polyacrylamide gel electrophoresis to facilitate analysis of giant myofibrillar proteins connectin and nebulin, whose molecular masses are about 3000 and 700 kDa, respectively. This method consists of 2% polyacrylamide slab gels strengthened with agarose and a specific buffer system. Our 2% polyacrylamide slab gels are superior to usual 1.8 or 2% polyacrylamide disc gels in preparation, handling, and resolution.

AB - We have established an improved method of sodium dodecyl sulfate-polyacrylamide gel electrophoresis to facilitate analysis of giant myofibrillar proteins connectin and nebulin, whose molecular masses are about 3000 and 700 kDa, respectively. This method consists of 2% polyacrylamide slab gels strengthened with agarose and a specific buffer system. Our 2% polyacrylamide slab gels are superior to usual 1.8 or 2% polyacrylamide disc gels in preparation, handling, and resolution.

UR - http://www.scopus.com/inward/record.url?scp=0028855384&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028855384&partnerID=8YFLogxK

U2 - 10.1006/abio.1995.1004

DO - 10.1006/abio.1995.1004

M3 - Article

C2 - 7710083

AN - SCOPUS:0028855384

VL - 224

SP - 28

EP - 31

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -