Detection of Haemophilus influenzae by loop-mediated isothermal amplification (LAMP) of the outer membrane protein P6 gene

Hirotaka Torigoe, Mitsuko Seki, Yoshihisa Yamashita, Atsuto Sugaya, Masao Maeno

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

It is difficult and time-consuming to distinguish Haemophilus influenzae from the genotypically similar Haemophilus parainfluenzae, which is a commensal of the human oral cavity. The novel nucleic acid amplification technique of loop-mediated isothermal amplification (LAW), which amplifies DNA under isothermal conditions (63°C) with high specificity, efficiency, and rapidity, was evaluated for H. influenzae detection. A H. influenzae-specific LAMP primer set was designed for the outer membrane protein P6 gene. Primer set specificity was validated using 4 Haemophilus spp. and 13 other species. Within 60 min, LAMP detected 100 or more copies of purified DNA with a sensitivity that was 10-fold higher than that of conventional PCR. This method can be used to differentiate H. influenzae from H. parainfluenzae strains. Thus, LAMP may represent a sensitive and reliable means of diagnosing H. influenzae infection.

Original languageEnglish
Pages (from-to)55-58
Number of pages4
JournalJapanese Journal of Infectious Diseases
Volume60
Issue number1
Publication statusPublished - Mar 26 2007

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

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