Detection of hematopoietic stem cells in microscopy images using a bank of ring filters

Sungeun Eom, Ryoma Bise, Takeo Kanade

Research output: Chapter in Book/Report/Conference proceedingConference contribution

11 Citations (Scopus)

Abstract

We present a method for robustly detecting hematopoietic stem cells (HSCs) in phase contrast microscopy images. HSCs appear to be easy to detect since they typically appear as round objects. However, when HSCs are touching and overlapping, showing the variations in shape and appearance, standard pattern detection methods, such as Hough transform and correlation, do not perform well. The proposed method exploits the output pattern of a ring filter bank applied to the input image, which consists of a series of matched filters with multiple-radius ring-shaped templates. By modeling the profile of each filter response as a quadratic surface, we explore the variations of peak curvatures and peak values of the filter responses when the ring radius varies. The method is validated on thousands of phase contrast microscopy images with different acquisition settings, achieving 96.5% precision and 94.4% recall.

Original languageEnglish
Title of host publication2010 7th IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro, ISBI 2010 - Proceedings
Pages137-140
Number of pages4
DOIs
Publication statusPublished - 2010
Externally publishedYes
Event7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Rotterdam, Netherlands
Duration: Apr 14 2010Apr 17 2010

Publication series

Name2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings

Other

Other7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010
CountryNetherlands
CityRotterdam
Period4/14/104/17/10

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

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