Detection of human papillomavirus types 16 and 18 in primary and in metastatic lesions of cervical carcinomas

Mansuka Mvula, Tsuyoshi Iwasaka, Norihito Matsuo, Yoshifumi Nakao, Tsunehisa Kaku, Toru Hachisuga, Kouichi Fukuda, Naoki Tsukamoto, Hajime Sugimori

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18 Citations (Scopus)


Human papillomavirus (HPV) types 16 and 18 are most prevalent in cervical carcinomas and are also present in metastatic sites. Thirty-six patients with lymph node metastases were examined for the possible presence of HPV 16 and 18 DNA sequences in the primary tumors as well as in metastatic lymph nodes. Polymerase chain reaction (PCR) analysis revealed the presence of the genome of HPV type 16 (HPV 16) in 17 tumors (47%) and that of HPV type 18 (HPV 18) in 2 tumors (6%), while 17 tumors (47%) were negative for both types. Of the 17 HPV 16-positive patients, 15 metastatic lymph nodes were also positive and 2 were negative. In 2 patients positive for HPV 18, the metastatic sites were negative. All the 17 patients negative for HPV in the primary tumors were also negative for HPV 16 and HPV 18 in the metastatic lymph nodes. These data confirmed the positive correlation of HPV DNA status between primary and metastatic tumors. However, the discrepancy in 11% of cases was attributed to presence of heterogeneous clones in the primary tumor.

Original languageEnglish
Pages (from-to)156-160
Number of pages5
JournalGynecologic Oncology
Issue number2
Publication statusPublished - Apr 1994

All Science Journal Classification (ASJC) codes

  • Oncology
  • Obstetrics and Gynaecology


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