Detection of interferon-γ-inducible chemokines in human milk

Yasushi Takahata, H. Takada, A. Nomura, H. Nakayama, K. Ohshima, T. Hara

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Aim: To assess the immunological role of human milk by analysing the concentrations of interferon-γ-inducible protein of 10 kda (IP-10) and monokine induced by interferon-γ (MIG) in human milk from mothers of preterm and term infants. Methods: IP-10 and MIG levels of colostrum, early milk, mature milk and sera were measured by enzyme-linked immunosorbent assay (ELISA). IP-10 and MIG mRNA expression levels in cellular components of human milk were determined by RT-PCR. IP-10 and MIG protein expression in mammary gland tissues was analysed by immunohistochemistry. Results: Significant amounts of IP-10 and MIG were detected in human milk. The concentrations of IP-10 and MIG in colostrum and early milk were significantly higher than those of sera from healthy controls or lactating mothers. These chemokine concentrations in colostrum and early milk were significantly higher than those of mature milk. Premature delivery or pregnancy complications of mothers had no significant correlation with these chemokine concentrations in breast milk. There were significant correlations between MIG and interferon-γ (IFN-γ) or IP-10 levels (p < 0.001) in human milk. Expression of IP-10 and MIG genes and proteins in the milk cells as well as in mammary gland epithelial tissues was detected by RT-PCR and immunohistochemistry. Conclusion: IP-10 and MIG in human milk, probably derived from milk cells and mammary gland epithelial cells, may contribute to the migration and activation of intestinal T lymphocytes to enhance mucosal immunity during the early neonatal period.

Original languageEnglish
Pages (from-to)659-665
Number of pages7
JournalActa Paediatrica, International Journal of Paediatrics
Volume92
Issue number6
DOIs
Publication statusPublished - Jun 1 2003

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Human Milk
Chemokines
Interferons
Milk
Colostrum
Human Mammary Glands
Mothers
Immunohistochemistry
Chemokine CXCL10
Monokines
Polymerase Chain Reaction
Mucosal Immunity
Pregnancy Complications
Serum
Premature Infants
Proteins
Epithelium
Epithelial Cells
Enzyme-Linked Immunosorbent Assay
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health

Cite this

Takahata, Y., Takada, H., Nomura, A., Nakayama, H., Ohshima, K., & Hara, T. (2003). Detection of interferon-γ-inducible chemokines in human milk. Acta Paediatrica, International Journal of Paediatrics, 92(6), 659-665. https://doi.org/10.1080/08035250310002614

Detection of interferon-γ-inducible chemokines in human milk. / Takahata, Yasushi; Takada, H.; Nomura, A.; Nakayama, H.; Ohshima, K.; Hara, T.

In: Acta Paediatrica, International Journal of Paediatrics, Vol. 92, No. 6, 01.06.2003, p. 659-665.

Research output: Contribution to journalArticle

Takahata, Y, Takada, H, Nomura, A, Nakayama, H, Ohshima, K & Hara, T 2003, 'Detection of interferon-γ-inducible chemokines in human milk', Acta Paediatrica, International Journal of Paediatrics, vol. 92, no. 6, pp. 659-665. https://doi.org/10.1080/08035250310002614
Takahata, Yasushi ; Takada, H. ; Nomura, A. ; Nakayama, H. ; Ohshima, K. ; Hara, T. / Detection of interferon-γ-inducible chemokines in human milk. In: Acta Paediatrica, International Journal of Paediatrics. 2003 ; Vol. 92, No. 6. pp. 659-665.
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