Detection of magnetic nanoparticles with superconducting quantum interference device (SQUID) magnetometer and application to immunoassays

Keiji Enpuku, Tadashi Minotani, Takemitsu Gima, Yukinori Kuroki, Yuzuru Itoh, Makiko Yamashita, Yoshinori Katakura, Satoru Kuhara

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

A system is developed to magnetically measure biological antigen-antibody reactions with a superconducting quantum interference device (SQUID) magnetometer. In this system, antibodies are labeled with magnetic nanoparticles of γ-Fe2O3, and the antigen-antibody reactions are measured by detecting the magnetic field from the magnetic nanoparticles. A setup of the system is described, and the sensitivity of the system is studied in terms of detectable weight of nanoparticles. Magnetic particles as small as 600 pg can be detected at present. An experiment is also conducted to measure antigen-antibody reaction with the present system. It is shown that the sensitivity of the present system is better than that of the conventional optical method. A one order of magnitude improvement of sensitivity will be realized by the sophistication of the present system.

Original languageEnglish
JournalJapanese Journal of Applied Physics, Part 2: Letters
Volume38
Issue number10 A
Publication statusPublished - Dec 1 1999

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Antigen-antibody reactions
immunoassay
SQUIDs
Magnetometers
antibodies
magnetometers
antigens
Nanoparticles
interference
nanoparticles
sensitivity
Antibodies
Magnetic fields
optics
Experiments
magnetic fields

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Physics and Astronomy (miscellaneous)
  • Physics and Astronomy(all)

Cite this

Detection of magnetic nanoparticles with superconducting quantum interference device (SQUID) magnetometer and application to immunoassays. / Enpuku, Keiji; Minotani, Tadashi; Gima, Takemitsu; Kuroki, Yukinori; Itoh, Yuzuru; Yamashita, Makiko; Katakura, Yoshinori; Kuhara, Satoru.

In: Japanese Journal of Applied Physics, Part 2: Letters, Vol. 38, No. 10 A, 01.12.1999.

Research output: Contribution to journalArticle

Enpuku, Keiji ; Minotani, Tadashi ; Gima, Takemitsu ; Kuroki, Yukinori ; Itoh, Yuzuru ; Yamashita, Makiko ; Katakura, Yoshinori ; Kuhara, Satoru. / Detection of magnetic nanoparticles with superconducting quantum interference device (SQUID) magnetometer and application to immunoassays. In: Japanese Journal of Applied Physics, Part 2: Letters. 1999 ; Vol. 38, No. 10 A.
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