Detection of Micrometastases in Sentinel Lymph Nodes of Melanoma Patients by RT-PCR

Yoichi Moroi, Chikage Obata, Shohei Fujita, Satoko Shibata, Sawako Konishi, Hong Duan, Shiori Saigo, Shuji Fukagawa, Akari Tashiro, Mito Toyota, Kenichi Yanagihara, Kazunori Urabe, Tetsuya Koga, Masutaka Furue

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Although biopsy of sentinel lymph nodes (SLNs) has become a standard procedure for patients with melanomas, detection of micrometastases in SLNs is still difficult. To overcome this problem, we have been using RT-PCR for detection of micrometastases in SLNs. Metastatic melanoma cells in an SLN are identified with very high sensivity by RT-PCR by using primer sets of melanoma-specific antigens, such as tyrosinase, MART-1 and gp100. Histopathological re-examination is required when RT-PCR reveals positive results, because biological false-positive results are relatively frequent in RT-PCR detection of micrometastasis.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalNishinihon Journal of Dermatology
Volume64
Issue number2
DOIs
Publication statusPublished - 2002

All Science Journal Classification (ASJC) codes

  • Dermatology

Fingerprint Dive into the research topics of 'Detection of Micrometastases in Sentinel Lymph Nodes of Melanoma Patients by RT-PCR'. Together they form a unique fingerprint.

  • Cite this

    Moroi, Y., Obata, C., Fujita, S., Shibata, S., Konishi, S., Duan, H., Saigo, S., Fukagawa, S., Tashiro, A., Toyota, M., Yanagihara, K., Urabe, K., Koga, T., & Furue, M. (2002). Detection of Micrometastases in Sentinel Lymph Nodes of Melanoma Patients by RT-PCR. Nishinihon Journal of Dermatology, 64(2), 211-217. https://doi.org/10.2336/nishinihonhifu.64.211