Detection of Micrometastases in Sentinel Lymph Nodes of Melanoma Patients by RT-PCR

Yoichi Moroi, Chikage Obata, Shohei Fujita, Satoko Shibata, Sawako Konishi, Hong Duan, Shiori Saigo, Shuji Fukagawa, Akari Tashiro, Mito Toyota, Kenichi Yanagihara, Kazunori Urabe, Tetsuya Koga, Masutaka Furue

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Although biopsy of sentinel lymph nodes (SLNs) has become a standard procedure for patients with melanomas, detection of micrometastases in SLNs is still difficult. To overcome this problem, we have been using RT-PCR for detection of micrometastases in SLNs. Metastatic melanoma cells in an SLN are identified with very high sensivity by RT-PCR by using primer sets of melanoma-specific antigens, such as tyrosinase, MART-1 and gp100. Histopathological re-examination is required when RT-PCR reveals positive results, because biological false-positive results are relatively frequent in RT-PCR detection of micrometastasis.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalNishinihon Journal of Dermatology
Volume64
Issue number2
DOIs
Publication statusPublished - Jan 1 2002

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Neoplasm Micrometastasis
Melanoma
Polymerase Chain Reaction
Melanoma-Specific Antigens
Sentinel Lymph Node Biopsy
Monophenol Monooxygenase
Sentinel Lymph Node

All Science Journal Classification (ASJC) codes

  • Dermatology

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Detection of Micrometastases in Sentinel Lymph Nodes of Melanoma Patients by RT-PCR. / Moroi, Yoichi; Obata, Chikage; Fujita, Shohei; Shibata, Satoko; Konishi, Sawako; Duan, Hong; Saigo, Shiori; Fukagawa, Shuji; Tashiro, Akari; Toyota, Mito; Yanagihara, Kenichi; Urabe, Kazunori; Koga, Tetsuya; Furue, Masutaka.

In: Nishinihon Journal of Dermatology, Vol. 64, No. 2, 01.01.2002, p. 211-217.

Research output: Contribution to journalArticle

Moroi, Y, Obata, C, Fujita, S, Shibata, S, Konishi, S, Duan, H, Saigo, S, Fukagawa, S, Tashiro, A, Toyota, M, Yanagihara, K, Urabe, K, Koga, T & Furue, M 2002, 'Detection of Micrometastases in Sentinel Lymph Nodes of Melanoma Patients by RT-PCR', Nishinihon Journal of Dermatology, vol. 64, no. 2, pp. 211-217. https://doi.org/10.2336/nishinihonhifu.64.211
Moroi, Yoichi ; Obata, Chikage ; Fujita, Shohei ; Shibata, Satoko ; Konishi, Sawako ; Duan, Hong ; Saigo, Shiori ; Fukagawa, Shuji ; Tashiro, Akari ; Toyota, Mito ; Yanagihara, Kenichi ; Urabe, Kazunori ; Koga, Tetsuya ; Furue, Masutaka. / Detection of Micrometastases in Sentinel Lymph Nodes of Melanoma Patients by RT-PCR. In: Nishinihon Journal of Dermatology. 2002 ; Vol. 64, No. 2. pp. 211-217.
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