Detection of monocyte chemoattractant protein-1 receptor expression in experimental atherosclerotic lesions: An autoradiographic study

Katsuichi Ohtsuki, Motoya Hayase, Koichi Akashi, Susan Kopiwoda, H. William Strauss

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Background - Monocytes, a common component of atheroma, are attracted to the lesion site in response to chemotactic signals, particularly expression of monocyte chemoattractant peptide 1 (MCP-1). This study assessed the feasibility of using radiolabeled MCP-1 to identify monocytes and macrophages that have localized at sites of experimental arterial lesions. Methods and Results - The biodistribution of radiolabeled MCP-1 was determined in normal mice, and localization in experimental atheroma was determined in cholesterol-fed rabbits 4 weeks after arterial injury of the iliac artery (9 rabbits) and the abdominal aorta (1 rabbit). Vessels were harvested and autoradiographed after intravenous administration of 125I-labeled MCP-1 and Evans blue dye. The arteries were evaluated histologically by hematoxylin and eosin staining and immune staining with a monoclonal antibody specific for rabbit macrophages (RAM-11). 125I-MCP-1 has a blood clearance half-time of ≈ 10 minutes and circulates in association with cells. The liver, lungs, and kidneys had the highest concentration of 125I-MCP-1 at 5 and 30 minutes after tracer administration. Autoradiograms revealed accumulation of 125I-MCP-1 in the damaged artery wall, with an average ratio of lesion to normal vessel of 6:1 (maximum 45:1). The accumulation of 125I-MCP-1 in the reendothelialized (plaque formation) areas was greater than in the deendothelialized (Evans blue-positive) areas (6.55±2.26 versus 4.34±1.43 counts/pixel, P<0.05). The uptake of 125I-MCP-1 correlated with the number of macrophages per unit area (r=0.85, P<0.0001). Conclusions - Radiolabeled MCP-1 may be a useful tracer for imaging monocyte/macrophage-rich experimental atherosclerotic lesions.

Original languageEnglish
Pages (from-to)203-208
Number of pages6
JournalCirculation
Volume104
Issue number2
DOIs
Publication statusPublished - Jul 10 2001
Externally publishedYes

Fingerprint

CCR2 Receptors
Chemotactic Factors
Monocytes
Peptides
Macrophages
Rabbits
Evans Blue
Atherosclerotic Plaques
Arteries
Staining and Labeling
Iliac Artery
Abdominal Aorta
Feasibility Studies
Hematoxylin
Eosine Yellowish-(YS)

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Detection of monocyte chemoattractant protein-1 receptor expression in experimental atherosclerotic lesions : An autoradiographic study. / Ohtsuki, Katsuichi; Hayase, Motoya; Akashi, Koichi; Kopiwoda, Susan; Strauss, H. William.

In: Circulation, Vol. 104, No. 2, 10.07.2001, p. 203-208.

Research output: Contribution to journalArticle

Ohtsuki, Katsuichi ; Hayase, Motoya ; Akashi, Koichi ; Kopiwoda, Susan ; Strauss, H. William. / Detection of monocyte chemoattractant protein-1 receptor expression in experimental atherosclerotic lesions : An autoradiographic study. In: Circulation. 2001 ; Vol. 104, No. 2. pp. 203-208.
@article{ede067040f9f45d881d510377324aa5a,
title = "Detection of monocyte chemoattractant protein-1 receptor expression in experimental atherosclerotic lesions: An autoradiographic study",
abstract = "Background - Monocytes, a common component of atheroma, are attracted to the lesion site in response to chemotactic signals, particularly expression of monocyte chemoattractant peptide 1 (MCP-1). This study assessed the feasibility of using radiolabeled MCP-1 to identify monocytes and macrophages that have localized at sites of experimental arterial lesions. Methods and Results - The biodistribution of radiolabeled MCP-1 was determined in normal mice, and localization in experimental atheroma was determined in cholesterol-fed rabbits 4 weeks after arterial injury of the iliac artery (9 rabbits) and the abdominal aorta (1 rabbit). Vessels were harvested and autoradiographed after intravenous administration of 125I-labeled MCP-1 and Evans blue dye. The arteries were evaluated histologically by hematoxylin and eosin staining and immune staining with a monoclonal antibody specific for rabbit macrophages (RAM-11). 125I-MCP-1 has a blood clearance half-time of ≈ 10 minutes and circulates in association with cells. The liver, lungs, and kidneys had the highest concentration of 125I-MCP-1 at 5 and 30 minutes after tracer administration. Autoradiograms revealed accumulation of 125I-MCP-1 in the damaged artery wall, with an average ratio of lesion to normal vessel of 6:1 (maximum 45:1). The accumulation of 125I-MCP-1 in the reendothelialized (plaque formation) areas was greater than in the deendothelialized (Evans blue-positive) areas (6.55±2.26 versus 4.34±1.43 counts/pixel, P<0.05). The uptake of 125I-MCP-1 correlated with the number of macrophages per unit area (r=0.85, P<0.0001). Conclusions - Radiolabeled MCP-1 may be a useful tracer for imaging monocyte/macrophage-rich experimental atherosclerotic lesions.",
author = "Katsuichi Ohtsuki and Motoya Hayase and Koichi Akashi and Susan Kopiwoda and Strauss, {H. William}",
year = "2001",
month = "7",
day = "10",
doi = "10.1161/01.CIR.104.2.203",
language = "English",
volume = "104",
pages = "203--208",
journal = "Circulation",
issn = "0009-7322",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Detection of monocyte chemoattractant protein-1 receptor expression in experimental atherosclerotic lesions

T2 - An autoradiographic study

AU - Ohtsuki, Katsuichi

AU - Hayase, Motoya

AU - Akashi, Koichi

AU - Kopiwoda, Susan

AU - Strauss, H. William

PY - 2001/7/10

Y1 - 2001/7/10

N2 - Background - Monocytes, a common component of atheroma, are attracted to the lesion site in response to chemotactic signals, particularly expression of monocyte chemoattractant peptide 1 (MCP-1). This study assessed the feasibility of using radiolabeled MCP-1 to identify monocytes and macrophages that have localized at sites of experimental arterial lesions. Methods and Results - The biodistribution of radiolabeled MCP-1 was determined in normal mice, and localization in experimental atheroma was determined in cholesterol-fed rabbits 4 weeks after arterial injury of the iliac artery (9 rabbits) and the abdominal aorta (1 rabbit). Vessels were harvested and autoradiographed after intravenous administration of 125I-labeled MCP-1 and Evans blue dye. The arteries were evaluated histologically by hematoxylin and eosin staining and immune staining with a monoclonal antibody specific for rabbit macrophages (RAM-11). 125I-MCP-1 has a blood clearance half-time of ≈ 10 minutes and circulates in association with cells. The liver, lungs, and kidneys had the highest concentration of 125I-MCP-1 at 5 and 30 minutes after tracer administration. Autoradiograms revealed accumulation of 125I-MCP-1 in the damaged artery wall, with an average ratio of lesion to normal vessel of 6:1 (maximum 45:1). The accumulation of 125I-MCP-1 in the reendothelialized (plaque formation) areas was greater than in the deendothelialized (Evans blue-positive) areas (6.55±2.26 versus 4.34±1.43 counts/pixel, P<0.05). The uptake of 125I-MCP-1 correlated with the number of macrophages per unit area (r=0.85, P<0.0001). Conclusions - Radiolabeled MCP-1 may be a useful tracer for imaging monocyte/macrophage-rich experimental atherosclerotic lesions.

AB - Background - Monocytes, a common component of atheroma, are attracted to the lesion site in response to chemotactic signals, particularly expression of monocyte chemoattractant peptide 1 (MCP-1). This study assessed the feasibility of using radiolabeled MCP-1 to identify monocytes and macrophages that have localized at sites of experimental arterial lesions. Methods and Results - The biodistribution of radiolabeled MCP-1 was determined in normal mice, and localization in experimental atheroma was determined in cholesterol-fed rabbits 4 weeks after arterial injury of the iliac artery (9 rabbits) and the abdominal aorta (1 rabbit). Vessels were harvested and autoradiographed after intravenous administration of 125I-labeled MCP-1 and Evans blue dye. The arteries were evaluated histologically by hematoxylin and eosin staining and immune staining with a monoclonal antibody specific for rabbit macrophages (RAM-11). 125I-MCP-1 has a blood clearance half-time of ≈ 10 minutes and circulates in association with cells. The liver, lungs, and kidneys had the highest concentration of 125I-MCP-1 at 5 and 30 minutes after tracer administration. Autoradiograms revealed accumulation of 125I-MCP-1 in the damaged artery wall, with an average ratio of lesion to normal vessel of 6:1 (maximum 45:1). The accumulation of 125I-MCP-1 in the reendothelialized (plaque formation) areas was greater than in the deendothelialized (Evans blue-positive) areas (6.55±2.26 versus 4.34±1.43 counts/pixel, P<0.05). The uptake of 125I-MCP-1 correlated with the number of macrophages per unit area (r=0.85, P<0.0001). Conclusions - Radiolabeled MCP-1 may be a useful tracer for imaging monocyte/macrophage-rich experimental atherosclerotic lesions.

UR - http://www.scopus.com/inward/record.url?scp=0035838375&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035838375&partnerID=8YFLogxK

U2 - 10.1161/01.CIR.104.2.203

DO - 10.1161/01.CIR.104.2.203

M3 - Article

C2 - 11447087

AN - SCOPUS:0035838375

VL - 104

SP - 203

EP - 208

JO - Circulation

JF - Circulation

SN - 0009-7322

IS - 2

ER -