TY - JOUR
T1 - Detection of SARS-CoV-2 Gene by Microbeads Dielectrophoresis-based DNA Detection Method
AU - Nakano, Michihiko
AU - Inaba, Masafumi
AU - Suehiro, Junya
N1 - Publisher Copyright:
© 2021 The Institute of Electrical Engineers of Japan.
PY - 2021
Y1 - 2021
N2 - Nucleic amplification tests (NATs) are sensitive and specific methods to diagnose infectious diseases. Real-time polymerase chain reaction (PCR) is the gold standard, but it requires expensive apparatus and regents because of fluorescent detection. This study performed a combination of a conventional PCR and microbeads dielectrophoresis (DEP)-based DNA detection method to detect the SARS-CoV-2 gene quantitatively. SARS-CoV-2 is a virus causing COVID-19 and has caused the pandemic worldwide. In the microbeads DEP-based DNA detection method, the amplified DNA, amplicon, is attached to microbeads, the amplicon-labeled microbeads are detected by dielectrophoretic impedance measurement. As a result, the technique demonstrated that same sensitivity as that of real-time PCR. Our method enables the cheap diagnosis of infectious disease instead of expensive real-time PCR.
AB - Nucleic amplification tests (NATs) are sensitive and specific methods to diagnose infectious diseases. Real-time polymerase chain reaction (PCR) is the gold standard, but it requires expensive apparatus and regents because of fluorescent detection. This study performed a combination of a conventional PCR and microbeads dielectrophoresis (DEP)-based DNA detection method to detect the SARS-CoV-2 gene quantitatively. SARS-CoV-2 is a virus causing COVID-19 and has caused the pandemic worldwide. In the microbeads DEP-based DNA detection method, the amplified DNA, amplicon, is attached to microbeads, the amplicon-labeled microbeads are detected by dielectrophoretic impedance measurement. As a result, the technique demonstrated that same sensitivity as that of real-time PCR. Our method enables the cheap diagnosis of infectious disease instead of expensive real-time PCR.
UR - http://www.scopus.com/inward/record.url?scp=85109207979&partnerID=8YFLogxK
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U2 - 10.1541/ieejsmas.141.233
DO - 10.1541/ieejsmas.141.233
M3 - Article
AN - SCOPUS:85109207979
VL - 141
SP - 233
EP - 236
JO - IEEJ Transactions on Sensors and Micromachines
JF - IEEJ Transactions on Sensors and Micromachines
SN - 1341-8939
IS - 7
ER -